Wang Peikun, Li Min, Li Haijuan, Bi Yuyu, Lin Lulu, Shi Mengya, Huang Teng, Mo Meilan, Wei Tianchao, Wei Ping
Institute for Poultry Science and Health, Guangxi University, Nanning 530004, China; Institute of Microbe and Host Health, Linyi University, Linyi, Shandong 276005, China.
Institute for Poultry Science and Health, Guangxi University, Nanning 530004, China.
Poult Sci. 2021 Apr;100(4):101027. doi: 10.1016/j.psj.2021.101027. Epub 2021 Jan 26.
One avian leukosis virus of subgroup J (ALV-J) strain GX14YYA1 was isolated from a commercial bivalent Newcastle disease (ND)-infectious bronchitis (IB) vaccine in our previous study. To evaluate the pathogenicity of the ALV-J-contaminated vaccine on commercial chickens, day-old Three-Yellow chicks in group I were vaccinated with ALV-J-contaminated bivalent ND-IB live vaccine by intranasal and eye drop at 1-day-old for the primary vaccination and at 7-day-old for the secondary vaccination. Groups II and III were kept as the normal vaccination group with the noncontaminated ND-IB vaccine and blank control groups, respectively. The birds of different groups were maintained separately in isolators for 175 d. The first viremia was detected at 4 wk of age and 20% (2/10) of the birds maintained viremia during 11 to 25 wk of age. At the same time, the birds in group I experienced a significant suppression of body weight gain when compared with those of groups II and III (P < 0.05). In addition, the birds in group I showed obvious ALV-J hemangioma-type anatomical lesions in the liver and tumors were observed in the abdominal cavity. The results demonstrated that the ALV-J contaminated commercial live vaccines can induce pathogenicity in commercial Three-Yellow chickens and indicate that ALV-J-contaminated commercial live vaccines could be one of the transmission routes of ALV-J to commercial chickens.
在我们之前的研究中，从一种商业化的新城疫（ND）-传染性支气管炎（IB）二价疫苗中分离出一株J亚群禽白血病病毒（ALV-J）GX14YYA1毒株。为评估被ALV-J污染的疫苗对商品鸡的致病性，I组1日龄三黄雏鸡在1日龄时通过滴鼻和点眼接种被ALV-J污染的二价ND-IB活疫苗进行首次免疫，7日龄时进行二次免疫。II组和III组分别作为接种未污染的ND-IB疫苗的正常免疫组和空白对照组。不同组的鸡分别在隔离器中饲养175天。在4周龄时检测到首次病毒血症，在11至25周龄期间，20%（2/10）的鸡维持病毒血症。同时，与II组和III组相比，I组鸡的体重增加受到显著抑制（P<0.05）。此外，I组鸡在肝脏出现明显的ALV-J血管瘤型解剖学病变，并且在腹腔中观察到肿瘤。结果表明，被ALV-J污染的商业化活疫苗可在商品三黄鸡中诱导致病性，并表明被ALV-J污染的商业化活疫苗可能是ALV-J传播给商品鸡的途径之一。
