Unidad de Recursos Forestales, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), Avda. Montañana 930, 50059 Zaragoza, Spain; Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza), Zaragoza, Spain.
University of Monastir, High Institute of Biotechnology of Monastir, Genetics, Biodiversity and Valorization of Bioresources Laboratory, Street Tahar Haddad, 5000 Monastir, Tunisia.
Food Res Int. 2021 Feb;140:110071. doi: 10.1016/j.foodres.2020.110071. Epub 2020 Dec 28.
Ten truffle species of Tuber and Terfezia genera were chemical characterized, assessing their proximate composition, individual nutrient compounds and some bioactive molecules. The bioactive properties of these species were also evaluated, namely their antioxidant, antimicrobial and cytotoxic potential. Carbohydrates were the main macronutrients present in truffles, followed by proteins. Furthermore, the levels of polyunsaturated fatty acids (PUFA), subsequently presented as a percentage, were higher in truffles (38.2-79.3%) except in Tuber magnatum and Terfezia arenaria, which have a more saturated fatty acids (SFA) profile (70.7% and 53.7%, respectively). Comparing the species, T. magnatum revealed the highest levels of total phenolic compounds (TPC) (290 mg GAE/100 g truffle), as also the best results in the four methods used to evaluate the antioxidant activity. On the other hand, only five extracts obtained from some studied truffle species (Terfezia magnusii, Tuber aestivum, Tuber gennadii, and Tuber melanosporum) showed a slight inhibition of microbial growth, tested against different bacteria. Terfezia and T. gennadii extracts, showed potential to inhibit the cellular growth of NCI-H460, HeLa, HepG2, and MCF-7 cell lines (GI concentrations range: 19-78, 33-301, 83-321 and 102-321 µg/mL, respectively), indicating anti-proliferative activity. Nevertheless, T. arenaria revealed some potential hepatotoxicity, inhibiting the growth of PLP2 cells (GI concentration of 220 µg/mL), a primary cell culture obtained from porcine liver.
十种块菌属（Tuber）和拟蜡块菌属（Terfezia）的块菌物种进行了化学特性分析，评估了它们的近似成分、个体营养化合物和一些生物活性分子。还评估了这些物种的生物活性特性，即它们的抗氧化、抗菌和细胞毒性潜力。碳水化合物是块菌中主要的宏量营养素，其次是蛋白质。此外，多不饱和脂肪酸（PUFA）的水平（随后以百分比表示）在块菌中较高（38.2-79.3%），除了 Tuber magnatum 和 Terfezia arenaria 外，它们的饱和脂肪酸（SFA）谱较高（分别为 70.7%和 53.7%）。比较这些物种，T. magnatum 显示出最高水平的总酚类化合物（TPC）（290mg GAE/100g 块菌），以及用于评估抗氧化活性的四种方法中的最佳结果。另一方面，只有从一些研究的块菌物种（Terfezia magnusii、Tuber aestivum、Tuber gennadii 和 Tuber melanosporum）中获得的五种提取物显示出对不同细菌生长的轻微抑制作用。Terfezia 和 T. gennadii 提取物显示出抑制 NCI-H460、HeLa、HepG2 和 MCF-7 细胞系细胞生长的潜力（GI 浓度范围：19-78、33-301、83-321 和 102-321µg/mL），表明具有抗增殖活性。然而，T. arenaria 显示出一些潜在的肝毒性，抑制 PLP2 细胞（GI 浓度为 220µg/mL）的生长，PLP2 细胞是从猪肝脏中获得的原代细胞培养物。
