在 LPS 诱导的神经炎症模型中 TSPO 和 MAO-B PET 放射性示踪剂的临床前评估
Preclinical Evaluation of TSPO and MAO-B PET Radiotracers in an LPS Model of Neuroinflammation.
机构信息
Azrieli Centre for Neuro-Radiochemistry, Brain Health Imaging Centre, Centre for Addiction and Mental Health, 250 College Street, Room 270, Toronto, Ontario M5T 1R8, Canada.
Brain Health Imaging Centre, Centre for Addiction and Mental Health, 250 College Street, Room 339, Toronto, Ontario M5T 1R8, Canada.
出版信息
PET Clin. 2021 Apr;16(2):233-247. doi: 10.1016/j.cpet.2020.12.003.
Discovery of novel PET radiotracers targeting neuroinflammation (microglia and astrocytes) is actively pursued. Employing a lipopolysaccharide (LPS) rat model, this longitudinal study evaluated the translocator protein 18-kDa radiotracer [F]FEPPA (primarily microglia) and monoamine oxidase B radiotracers [C]L-deprenyl and [C]SL25.1188 (astrocytes preferred). Increased [F]FEPPA binding peaked at 1 week in LPS-injected striatum whereas increased lazabemide-sensitive [C]L-deprenyl binding developed later. No increase in radiotracer uptake was observed for [C]SL25.1188. The unilateral intrastriatal LPS rat model may serve as a useful tool for benchmarking PET tracers targeted toward distinct phases of neuroinflammatory reactions involving both microglia and astrocytes.
目前正在积极探索针对神经炎症（小胶质细胞和星形胶质细胞）的新型正电子发射断层扫描（PET）示踪剂。本纵向研究采用脂多糖（LPS）大鼠模型，评估了转位蛋白 18 kDa 放射性示踪剂 [F]FEPPA（主要靶向小胶质细胞）和单胺氧化酶 B 放射性示踪剂 [C]L-Deprenyl 和 [C]SL25.1188（优先靶向星形胶质细胞）。LPS 注射纹状体中的 [F]FEPPA 结合增加在 1 周时达到峰值，而 lazabemide 敏感的 [C]L- Deprenyl 结合增加则较晚出现。[C]SL25.1188 的放射性示踪剂摄取没有增加。单侧纹状体 LPS 大鼠模型可能成为用于比较针对涉及小胶质细胞和星形胶质细胞的神经炎症反应不同阶段的不同 PET 示踪剂的有用工具。
Zotero 插件