Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, 100093, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Plant Mol Biol. 2021 Apr;105(6):585-599. doi: 10.1007/s11103-020-01111-x. Epub 2021 Mar 2.
Total of 14 SNPs associated with overwintering-related traits and 75 selective regions were detected. Important candidate genes were identified and a possible network of cold-stress responses in woody plants was proposed. Local adaptation to low temperature is essential for woody plants to against changeable climate and safely survive the winter. To uncover the specific molecular mechanism of low temperature adaptation in woody plants, we sequenced 134 core individuals selected from 494 paper mulberry (Broussonetia papyrifera), which naturally distributed in different climate zones and latitudes. The population structure analysis, PCA analysis and neighbor-joining tree analysis indicated that the individuals were classified into three clusters, which showed forceful geographic distribution patterns because of the adaptation to local climate. Using two overwintering phenotypic data collected at high latitudes of 40°N and one bioclimatic variable, genome-phenotype and genome-environment associations, and genome-wide scans were performed. We detected 75 selective regions which possibly undergone temperature selection and identified 14 trait-associated SNPs that corresponded to 16 candidate genes (including LRR-RLK, PP2A, BCS1, etc.). Meanwhile, low temperature adaptation was also supported by other three trait-associated SNPs which exhibiting significant differences in overwintering traits between alleles within three geographic groups. To sum up, a possible network of cold signal perception and responses in woody plants were proposed, including important genes that have been confirmed in previous studies while others could be key potential candidates of woody plants. Overall, our results highlighted the specific and complex molecular mechanism of low temperature adaptation and overwintering of woody plants.
共检测到与越冬相关性状相关的 14 个 SNP 和 75 个选择区域。鉴定了重要的候选基因，并提出了一个可能的木本植物冷应激反应网络。低温适应对于木本植物抵御气候变化和安全越冬至关重要。为了揭示木本植物低温适应的特定分子机制，我们对 134 个核心个体进行了测序，这些个体是从自然分布在不同气候带和纬度的 494 株构树（Broussonetia papyrifera）中选择的。种群结构分析、PCA 分析和邻接树分析表明，这些个体分为三个聚类，由于适应当地气候，表现出强烈的地理分布模式。利用在 40°N 高纬度收集的两个越冬表型数据和一个生物气候变量，进行了基因组-表型和基因组-环境关联以及全基因组扫描。我们检测到 75 个可能经历温度选择的选择区域，并鉴定了 14 个与性状相关的 SNP，这些 SNP 对应 16 个候选基因（包括 LRR-RLK、PP2A、BCS1 等）。同时，低温适应也得到了另外三个与性状相关的 SNP 的支持，这些 SNP 在三个地理群体内的等位基因之间的越冬性状表现出显著差异。总之，提出了一个木本植物冷信号感知和响应的可能网络，包括已在先前研究中得到证实的重要基因，以及其他可能是木本植物关键潜在候选基因。总的来说，我们的研究结果突出了木本植物低温适应和越冬的特定而复杂的分子机制。
