Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama 930-0194, Japan.
Department of Chemistry, University of Yangon, Yangon 11041, Myanmar.
Fitoterapia. 2021 Jun;151:104870. doi: 10.1016/j.fitote.2021.104870. Epub 2021 Feb 27.
Six new isopimarane diterpenoids, shanpanootols A-F (1-6), along with two known analogues, were isolated from the ethyl acetate-soluble extract of Kaempferia pulchra rhizomes collected in Myanmar. The structures of these compounds were elucidated by extensive spectroscopic techniques such as 1D and 2D NMR and HRESIMS. The absolute configuration of 1 was determined by the modified Mosher method. The new isolates (1-6) were tested for their Vpr inhibitory activities against TREx-HeLa-Vpr cells. Shanpanootols C (3) and E (5) inhibited Vpr at doses of 2.5 and 5 μM, respectively.
从缅甸产的山柰根状茎的乙酸乙酯可溶部分分离得到 6 个新的异贝壳杉烷二萜，即山柰醇 A-F（1-6），并结合 2D NMR 和 HRESIMS 等波谱技术鉴定了它们的结构。通过改进的 Mosher 方法确定了 1 的绝对构型。对新分离得到的化合物（1-6）进行了对 TREx-HeLa-Vpr 细胞的 Vpr 抑制活性测试。山柰醇 C（3）和 E（5）在 2.5 和 5 μM 的剂量下分别抑制 Vpr。
