Do Kiep Minh, Hoshino Shotaro, Kodama Takeshi, Nguyen Hien Minh, Van Le Son, Ikumi Naotaka, Onaka Hiroyasu, Morita Hiroyuki
Institute of Natural Medicine, University of Toyama, 2630-Sugitani, Toyama, 930-0194, Japan.
Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-Ku, Tokyo, 171-8588, Japan.
J Nat Med. 2024 Jun;78(3):537-546. doi: 10.1007/s11418-024-01789-z. Epub 2024 Mar 22.
A phytochemical investigation of Kaempferia champasakensis rhizomes led to the isolation of a new 3,4-seco-isopimarane diterpene, kaempferiol A (1), and three new isopimarane diterpenes, kaempferiols B-D (2-4), together with six known isopimarane diterpenes (5-10). The structures of 1-4 were elucidated by extensive spectroscopic analyses, including HR-ESI-MS, UV, IR, and 1D and 2D NMR. The absolute configurations of 1, 3, and 4 were determined by ECD calculations, while that of 2 was established using the modified Mosher method. All isolated compounds were tested for cytotoxicity against three human cancer cell lines, lung cancer (A549), cervical cancer (HeLa), and breast cancer (MCF-7). Among them, 6 and 7 showed moderate cytotoxic activities against the three tested cell lines, with IC values ranging from 38.04 to 27.77 μM, respectively.
对长柄山柰根茎进行植物化学研究，从中分离出一种新的3,4-裂环异海松烷二萜化合物山柰酚A（1）、三种新的异海松烷二萜化合物山柰酚B - D（2 - 4），以及六种已知的异海松烷二萜化合物（5 - 10）。通过高分辨电喷雾电离质谱（HR-ESI-MS）、紫外光谱（UV）、红外光谱（IR）以及一维和二维核磁共振谱等广泛的光谱分析手段，阐明了化合物1 - 4的结构。通过电子圆二色光谱（ECD）计算确定了化合物1、3和4的绝对构型，而化合物2的绝对构型则采用改良的莫舍尔方法确定。对所有分离得到的化合物进行了针对三种人类癌细胞系（肺癌（A549）、宫颈癌（HeLa）和乳腺癌（MCF-7））的细胞毒性测试。其中，化合物6和7对三种受试细胞系表现出中等程度的细胞毒性活性，其半数抑制浓度（IC）值分别在38.04至27.77 μM范围内。
