Savkovic Vuk, Li Hanluo, Obradovic Danilo, Masieri Federica Francesca, Bartella Alexander K, Zimmerer Rüdiger, Simon Jan-Christoph, Etz Christian, Lethaus Bernd
Department of Oral and Maxillofacial Surgery, Leipzig University Medical Center, 04103 Leipzig, Germany.
Department of Cardiology and Angiology, Leipzig Heart Center, 04289 Leipzig, Germany.
J Clin Med. 2021 Feb 26;10(5):911. doi: 10.3390/jcm10050911.
Neovascularization is regarded as a pre-requisite in successful tissue grafting of both hard and soft tissues alike. This study considers mesenchymal stem cells from hair follicle outer root sheath (MSCORS) as powerful tools with a neat angiogenic potential that could in the future have wide scopes of neo-angiogenesis and tissue engineering. Autologous MSCORS were obtained ex vivo by non-invasive plucking of hair and they were differentiated in vitro into both endothelial cells and vascular smooth muscle cells (SMCs), two crucial cellular components of vascular grafts. Assessment was carried out by immunostaining, confocal laser-scanning microscopy, gene expression analysis (qRT-PCR), quantitative analysis of anastomotic network parameters, and cumulative length quantification of immunostained α-smooth muscle actin-containing stress fibers (α -SMA). In comparison to adipose mesenchymal stem cells, MSCORS exhibited a significantly higher differentiation efficiency according to key quantitative criteria and their endothelial derivatives demonstrated a higher angiogenic potential. Furthermore, the cells were capable of depositing their own extracellular matrix in vitro in the form of a membrane-cell sheet, serving as a base for viable co-culture of endothelial cells and SMCs integrated with their autologous matrix. Differentiated MSCORS hereby provided a complex autologous cell-matrix construct that demonstrates vascularization capacity and can serve as a base for personalized repair grafting applications.
新生血管形成被视为成功进行软硬组织移植的一个先决条件。本研究认为毛囊外根鞘间充质干细胞（MSCORS）是具有强大血管生成潜力的有力工具，未来在新生血管形成和组织工程方面可能有广泛应用。通过非侵入性拔毛在体外获得自体MSCORS，并将其在体外分化为内皮细胞和血管平滑肌细胞（SMC），这是血管移植物的两个关键细胞成分。通过免疫染色、共聚焦激光扫描显微镜、基因表达分析（qRT-PCR）、吻合网络参数的定量分析以及免疫染色的含α平滑肌肌动蛋白应力纤维（α -SMA）的累积长度定量进行评估。与脂肪间充质干细胞相比，根据关键定量标准，MSCORS表现出显著更高的分化效率，其内皮衍生物表现出更高的血管生成潜力。此外，这些细胞能够在体外以膜细胞片的形式沉积自身的细胞外基质，作为与自身基质整合的内皮细胞和SMC可行共培养的基础。分化的MSCORS由此提供了一种复杂的自体细胞 - 基质构建体，其展示了血管化能力，并可作为个性化修复移植应用的基础。
