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黄芩苷对脂多糖刺激仔猪腹膜间皮细胞紧密连接的保护作用

Protective Effects of Baicalin on Peritoneal Tight Junctions in Piglets Challenged with .

机构信息

Hubei Key Laboratory of Animal Nutrition and Feed Science, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China.

Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.

出版信息

Molecules. 2021 Feb 26;26(5):1268. doi: 10.3390/molecules26051268.

DOI:10.3390/molecules26051268
PMID:33652818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7956672/
Abstract

() causes inflammation and damage to piglets. Whether polyserositis caused by is due to tight junctions damage and the protective effect of baicalin on it have not been examined. Therefore, this study aims to investigate the effects of baicalin on peritoneal tight junctions of piglets challenged with and its underlying molecular mechanisms. Piglets were challenged with and treated with or without baicalin. RT-PCR was performed to examine the expression of peritoneal tight junctions genes. Immunofluorescence was carried out to detect the distribution patterns of tight junctions proteins. Western blot assays were carried out to determine the involved signaling pathways. Our data showed that infection can down-regulate the tight junctions expression and disrupt the distribution of tight junctions proteins. Baicalin can alleviate the down-regulation of tight junctions mRNA in peritoneum, prevent the abnormalities and maintain the continuous organization of tight junctions. Our results provide novel evidence to support that baicalin has the capacity to protect peritoneal tight junctions from -induced inflammation. The protective mechanisms of baicalin could be associated with inhibition of the activation of PKC and MLCK/MLC signaling pathway. Taken together, these data demonstrated that baicalin is a promising natural agent for the prevention and treatment of infection.

摘要

（）可引起仔猪炎症和损伤。 是否由 引起的多发性浆膜炎是由于紧密连接损伤以及黄芩苷对其的保护作用尚未得到检验。 因此，本研究旨在探讨黄芩苷对感染猪腹腔紧密连接的影响及其潜在的分子机制。 将仔猪用 感染，并分别用或不用黄芩苷处理。 进行 RT-PCR 以检查腹膜紧密连接基因的表达。 进行免疫荧光染色以检测紧密连接蛋白的分布模式。 通过 Western blot 检测确定涉及的信号通路。 我们的数据表明，感染可下调紧密连接的表达并破坏紧密连接蛋白的分布。 黄芩苷可减轻腹膜中紧密连接 mRNA 的下调，防止异常并维持紧密连接的连续组织。 我们的结果提供了新的证据支持黄芩苷具有保护腹膜紧密连接免受 诱导的炎症的能力。 黄芩苷的保护机制可能与抑制 PKC 和 MLCK/MLC 信号通路的激活有关。 综上所述，这些数据表明黄芩苷是预防和治疗 感染的有前途的天然药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f80e/7956672/df8fac9b3fa2/molecules-26-01268-g008.jpg
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