Merwid-Ląd Anna, Ksiądzyna Dorota, Hałoń Agnieszka, Szkudlarek Danuta, Trocha Małgorzata, Szandruk-Bender Marta, Matuszewska Agnieszka, Nowak Beata, Sozański Tomasz, Kuźniar Anna, Szeląg Adam
Department of Pharmacology, Wroclaw Medical University, ul. Mikulicza-Radeckiego 2, 50-345 Wrocław, Poland.
Department of Pathomorphology and Oncological Cytology, Wroclaw Medical University, ul. Borowska 213, 50-556 Wrocław, Poland.
Pharmaceuticals (Basel). 2021 Feb 26;14(3):192. doi: 10.3390/ph14030192.
Cyclophosphamide (CPX) exerts toxicity in the urogenital system. The current study was designed to evaluate the effect of morin-5'-sulfonic acid sodium salt (NaMSA) on CPX-induced urogenital toxicity in rats. NaMSA (100 mg/kg/daily) and CPX (15 mg/kg/daily) alone or in combination and 0.9% NaCl (as a control) were given intragastrically for 10 days. Testes and epididymes from male and urinary bladders from male and female rats were evaluated histologically. In testes and epididymes, morphological changes and relative decrease in sperm count were assessed. In urinary bladders edema, hemorrhage and urothelium erosions were described by 0-2 points scoring system. Reproductive score (RS-in total 6 points) and urinary bladder score (BS-in total 6 points) were thereafter calculated. In CPX-receiving group RS (2.7) and BS (3.3) were significantly higher than in the control (0.5 and 0.25 for RS and BS, respectively). Co-administration of NaMSA reversed most of the morphological changes, which was reflected by lower RS and BS score (0.5 and 1.2 for RS and BS, respectively). The preliminary findings suggest that NaMSA may attenuate CPX-induced histological changes in rat urogenital tract.
环磷酰胺（CPX）对泌尿生殖系统具有毒性。本研究旨在评估桑色素 - 5'-磺酸钠盐（NaMSA）对CPX诱导的大鼠泌尿生殖毒性的影响。单独或联合给予NaMSA（100mg/kg/每日）和CPX（15mg/kg/每日），并给予0.9%氯化钠（作为对照）灌胃10天。对雄性大鼠的睾丸和附睾以及雄性和雌性大鼠的膀胱进行组织学评估。在睾丸和附睾中，评估形态学变化和精子计数的相对减少。在膀胱中，通过0 - 2分评分系统描述水肿、出血和尿路上皮糜烂情况。此后计算生殖评分（RS - 总分6分）和膀胱评分（BS - 总分6分）。在接受CPX的组中，RS（2.7）和BS（3.3）显著高于对照组（RS和BS分别为0.5和0.25）。NaMSA的联合给药逆转了大多数形态学变化，这通过较低的RS和BS评分反映出来（RS和BS分别为0.5和1.2）。初步研究结果表明，NaMSA可能减轻CPX诱导的大鼠泌尿生殖道组织学变化。
