Center for Behavioral Sciences and Mental Health, Italian National Institute of Health, 00161 Rome, Italy.
Department of Biological Sciences, Tor Vergata University, 00133 Rome, Italy.
Int J Environ Res Public Health. 2021 Feb 26;18(5):2322. doi: 10.3390/ijerph18052322.
Previous studies have shown multiple biological properties of , a plant native to Africa and Asia. In the present study, potential physiological properties of microvesicles extracted from seeds were assessed. For this purpose, we investigated behavioral profile and hematological parameters in a recent rat model characterized by dysregulation in dopamine transporter, a key regulator of dopaminergic system. Experimental design consisted of male Wistar-DAT rats aged between two and four months: wild-type (WT) ( = 5) and heterozygous (DATHET) ( = 4) control groups, which drank tap water; WT ( = 5) and DATHET ( = 6) groups which drank a solution of microvesicles and water (2: 68 mL per day), which was orally administered for two months. Rats were monitored for spontaneous locomotor activity on a 24/7 basis. In the early lit hours, treated DATHET subjects showed higher locomotor activity, proposing a sleep-delay effect of . In forced swimming test, WT subjects who took exhibited more depressive behavior. In DATHET rats, seemed to potentiate the struggle to find a way out, counteracting an initial panic. Hemoglobin and hematocrit underwent opposite changes in either genotype, supporting the opposite effects on behavioral phenotype observed. Future work is clearly needed to further explore these preliminary profiles.
先前的研究表明， ，一种原产于非洲和亚洲的植物，具有多种生物学特性。在本研究中，评估了从 种子中提取的微泡的潜在生理特性。为此，我们在最近的一种多巴胺转运体失调的大鼠模型中研究了行为特征和血液学参数，多巴胺转运体是多巴胺能系统的关键调节因子。实验设计包括年龄在 2 至 4 个月之间的雄性 Wistar-DAT 大鼠：野生型（WT）（ = 5）和杂合子（DATHET）（ = 4）对照组，饮用自来水；WT（ = 5）和 DATHET（ = 6）组饮用 微泡和水的溶液（每天 2：68 毫升），连续口服两个月。大鼠在 24/7 基础上监测自发运动活动。在早期光照时间，接受治疗的 DATHET 受检者表现出更高的运动活性，提出了 的睡眠延迟效应。在强迫游泳试验中，服用 的 WT 受检者表现出更多的抑郁行为。在 DATHET 大鼠中， 似乎增强了寻找出路的挣扎，抵消了最初的恐慌。无论基因型如何，血红蛋白和血细胞比容都发生了相反的变化，支持了对观察到的行为表型的相反影响。显然需要进一步探索这些初步特征。
