School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China.
The Second People's Hospital of Huadu District,Guangzhou 510320, China.
Int J Med Sci. 2021 Apr 3;18(11):2312-2320. doi: 10.7150/ijms.52575. eCollection 2021.
We explored has_circ_0071106 as a diagnostic marker for type 2 diabetes (T2DM) in south China, and predicted the functional mechanism of the target circRNA. A total of 107 T2DM patients and 107 healthy reference persons were included as the research objects. In the first stage, the circRNA microarray was used to detect the peripheral blood of 4 T2DM and 4 control groups to screen the differential expression profile of circRNA. In the second stage, four circRNAs were screened from the differential expression profiles of circRNA, and real-time polymerase chain reaction (Q-PCR) technology was used to verify the blood samples of 103 T2DM and 103 controls. Finally, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis in bioinformatics were used to predict the functional mechanism of target circRNA. Lastly,we found that has_circ_0071106 increase the risk of T2DM (OR=2.819 (95% CI: 1.415~5.616)). Thearea under the ROCcurve has_circ_0071106 was 0.690, the sensitivity was 62.1%, and the specificity was 69.9%. The function prediction results showed that has_circ_0071106 was involved in biological processes such as protein binding, gene transcription, and may be involved in the pathway of hsa-miR-29a-5p regulating diabetes, has_circ_0071106 may be used as a diagnostic marker for T2DM.
我们探讨了 has_circ_0071106 作为中国南方 2 型糖尿病（T2DM）的诊断标志物，并预测了靶 circRNA 的功能机制。共纳入 107 例 T2DM 患者和 107 例健康对照者作为研究对象。在第一阶段，使用 circRNA 微阵列检测 4 例 T2DM 和 4 例对照组的外周血，以筛选 circRNA 的差异表达谱。在第二阶段，从差异表达谱的 circRNA 中筛选出 4 个 circRNA，并用实时聚合酶链反应（Q-PCR）技术验证了 103 例 T2DM 和 103 例对照者的血液样本。最后，利用生物信息学中的基因本体（GO）和京都基因与基因组百科全书（KEGG）通路分析预测靶 circRNA 的功能机制。最后，我们发现 has_circ_0071106 增加了 T2DM 的风险（OR=2.819（95%CI：1.415~5.616））。ROC 曲线下的 has_circ_0071106 面积为 0.690，敏感性为 62.1%，特异性为 69.9%。功能预测结果表明，has_circ_0071106 参与了蛋白质结合、基因转录等生物学过程，可能参与了 hsa-miR-29a-5p 调节糖尿病的通路，has_circ_0071106 可能作为 T2DM 的诊断标志物。
