通过阿拉玛蓝法评估银纳米颗粒对A549人肺癌细胞系的抗癌活性。
Evaluation of Anticancer activity of Silver Nanoparticles on the A549 Human lung carcinoma cell lines through Alamar Blue Assay.
作者信息
Sharma Nikita, Arya Geeta, Kumari R Mankamna, Gupta Nidhi, Nimesh Surendra
机构信息
Department of Biotechnology, Central University of Rajasthan, Ajmer, India.
Department of Biotechnology, The IIS University, Gurukul Marg, SFS, Mansarovar, Jaipur, Rajasthan, India.
出版信息
Bio Protoc. 2019 Jan 5;9(1):e3131. doi: 10.21769/BioProtoc.3131.
Silver nanoparticles have been widely studied to possess antimicrobial as well as anticancer activity, and have found its applications in various fields including pharmaceutical industry, diagnostics, drug delivery, food industry, and others. For this purpose, several cell proliferation assays are widely used for the evaluation of anticancer activity of synthetic compounds as well as natural plant extracts. In general, a compound is said to possess an anticancer activity if it prevents the cancer cells to grow and divide actively, and indirectly activates the generic program of cell death. In this protocol, Alamar blue and MTT assay are described for the analysis of metabolic function and health of the cell. These procedures are generally used for the endpoint analysis. A549 cells are seeded in a 96-well plate, and after the adherence of the cells, they are treated with different concentrations of silver nanoparticles. Followed by 24 h of incubation, colorimetric dyes are added to the wells, and the absorbance is recorded to quantify the percentage cytotoxicity in the sample wells.
银纳米颗粒已被广泛研究，具有抗菌和抗癌活性，并已在包括制药行业、诊断、药物递送、食品工业等在内的各个领域得到应用。为此，几种细胞增殖测定法被广泛用于评估合成化合物以及天然植物提取物的抗癌活性。一般来说，如果一种化合物能阻止癌细胞活跃生长和分裂，并间接激活细胞死亡的一般程序，就说它具有抗癌活性。在本方案中，描述了用于分析细胞代谢功能和健康状况的alamar蓝和MTT测定法。这些程序通常用于终点分析。将A549细胞接种到96孔板中，细胞贴壁后，用不同浓度的银纳米颗粒处理。孵育24小时后，向孔中加入比色染料，并记录吸光度以量化样品孔中的细胞毒性百分比。
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