Shao Xiaolong, Xie Yingpeng, Zhang Yingchao, Deng Xin
Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin 300457, China.
Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong SAR, China.
Bio Protoc. 2019 May 20;9(10):e3237. doi: 10.21769/BioProtoc.3237.
is a model plant pathogen that infects more than 50 plant species worldwide, thus leading to significant yield loss. biofilm always adheres to the surfaces of medical devices or host cells, thereby contributing to infection. Biofilm formation can be visualized on numerous matrixes, including coverslips, silicone tubes, polypropylene and polystyrene. Confocal laser scanning microscopy can be used to visualize and analyze biofilm structure. In this study, we modified and applied the current method of biofilm measurement to , and developed a convenient protocol to visualize biofilm formation using a borosilicate glass tube as the matrix coupled with crystal violet staining.
是一种典型的植物病原体，在全球感染50多种植物，导致严重的产量损失。生物膜总是附着在医疗器械或宿主细胞表面，从而导致感染。生物膜形成可以在许多基质上观察到，包括盖玻片、硅胶管、聚丙烯和聚苯乙烯。共聚焦激光扫描显微镜可用于观察和分析生物膜结构。在本研究中，我们对当前生物膜测量方法进行了改进并应用于……，并开发了一种简便的方案，以硼硅酸盐玻璃管为基质结合结晶紫染色来观察生物膜形成。
