聚乙二醇介导的……的稳定核转化和叶绿体转化 （原文不完整，翻译至此）
PEG-mediated, Stable, Nuclear and Chloroplast Transformation of .
作者信息
Zienkiewicz Maksymilian, Krupnik Tomasz, Drożak Anna, Kania Kinga
机构信息
Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096, Warsaw, Poland.
出版信息
Bio Protoc. 2019 Sep 5;9(17):e3355. doi: 10.21769/BioProtoc.3355.
Abstract
The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been achieved in multiple species. Our newly developed method allows for relatively simple transformation of in both nuclear and chloroplast genome by means of homologous recombination between the genome and a transformation vector. The use of chloramphenicol resistance gene as the selectable marker allows for plate-based efficient selection of mutant colonies. Overall, the method allows the generation of mutant strains within 6 months.
摘要
实现植物细胞核或叶绿体转化的能力一直是一个长期目标，尤其是在微藻研究中。在过去的几年里，取得的成功寥寥无几，但已经在多个物种中实现了瞬时和稳定的细胞核转化。我们新开发的方法允许通过基因组与转化载体之间的同源重组，相对简单地对细胞核和叶绿体基因组进行转化。使用氯霉素抗性基因作为选择标记，可以基于平板高效筛选突变菌落。总体而言，该方法能够在6个月内生成突变菌株。
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