Bartolini Marco, Grau Roberto
Departamento de Microbiología, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, CONICET, Rosario, Argentina.
Bio Protoc. 2019 Nov 20;9(22):e3425. doi: 10.21769/BioProtoc.3425.
Surface-associate motility on biotic and abiotic environments is a key mechanism used by the model bacterium and its closest relatives () for surface colonization and spreading across surfaces. The study of this mechanism in a research, industrial or clinic laboratory is essential; however, precautions should be taken for the reproducibility of the results, for example, the procedure to inoculate the bacteria on the testing plate, the humidity of the plate and the agar concentration. In this protocol, we describe, using , how to perform these assays and, in addition, we show how by varying the agar concentration in the plate, you can make a first approximation of what type of motility has other bacterial species.
在生物和非生物环境中的表面相关运动是模式细菌及其近缘菌（）用于表面定殖和在表面扩散的关键机制。在研究、工业或临床实验室中研究这一机制至关重要；然而，应采取预防措施以确保结果的可重复性，例如，将细菌接种到测试平板上的程序、平板的湿度和琼脂浓度。在本方案中，我们描述了如何使用进行这些测定，此外，我们还展示了如何通过改变平板中的琼脂浓度，初步了解其他细菌物种具有何种类型的运动性。
