用于高效培养单细胞红藻的顶级淀粉平板法
Top Starch Plating Method for the Efficient Cultivation of Unicellular Red Alga .
作者信息
Takemura Tokiaki, Kobayashi Yuki, Imamura Sousuke, Tanaka Kan
机构信息
Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuta 4259-R1-29, Yokohama 226-8503, Japan.
School of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta 4259-R1-29, Yokohama 226-8503, Japan.
出版信息
Bio Protoc. 2019 Feb 20;9(4):e3172. doi: 10.21769/BioProtoc.3172.
Abstract
The unicellular red alga has been used as a model photosynthetic eukaryote for various basic and applied studies, and several of these molecular genetics techniques have been reported. However, there are still improvements to be made concerning the plating method. The conventional plating method often generates diffuse colonies and single colonies cannot be easily isolated. To overcome these problems, we established a novel plating method for , making use of melted cornstarch as the use of top agar plating in bacterial genetics. This method improved the formation of defined colonies in at least 4-fold higher efficiency than the conventional method, and made the handling procedure much easier than the previous method.
摘要
单细胞红藻已被用作各种基础和应用研究的模式光合真核生物，并且已经报道了其中一些分子遗传学技术。然而，关于平板接种方法仍有改进的空间。传统的平板接种方法常常产生扩散的菌落，难以轻易分离出单菌落。为了克服这些问题，我们建立了一种新的平板接种方法，利用融化的玉米淀粉，就像在细菌遗传学中使用顶层琼脂平板接种一样。这种方法使明确菌落的形成效率比传统方法提高了至少4倍，并且使操作过程比以前的方法更加简便。
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