Grupo de Materiais Poliméricos e Compósitos, Departamento de Química, Universidade Estadual de Maringá - UEM, Av. Colombo, 5790 - Zona 7, 87020-900 Maringá, PR, Brazil.
Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Departamento de Ciências Básicas da Saúde, UEM, Brazil.
Int J Biol Macromol. 2021 Jun 1;180:88-96. doi: 10.1016/j.ijbiomac.2021.02.194. Epub 2021 Feb 28.
Chitosan/chondroitin sulfate (CHT/CS) curcumin-charged hydrogels were prepared through polyelectrolytic complexation (PEC) following two methodologies (PEC-CUR and PEC-T-CUR) and were applied on apoptosis of HeLa, HT29 and PC3 cancer cells. PEC-T-CUR (ionic liquid (IL) mixed using ultraturrax homogenizer) results show to be far better than for PEC-CUR (IL mixed using magnetic stirring), with IC being improved 5.13 times to HeLa cancer cells (from 1675.2 to 326.7 μg mL). PECs produced by this methodology presented favorable characteristics, such as particle size, hydrophobicity, pH swelling. Beyond this, the IL was quantitatively recovered in both cases. CUR entrapment levels were hugely loaded into PEC at around 100%. Swelling, dissolution/degradation, and pH assays showed that PECs may positively act in several environments, releasing the CUR, the CHT and CS as well. Characterization through FTIR, SEM, TEM, TGA, DSC, and WAXS confirmed CUR presence in both types of PECs, and cytotoxic studies showed the significant anticancer effects of CUR-containing PECs.
壳聚糖/硫酸软骨素（CHT/CS）姜黄素负载水凝胶是通过聚电解质络合（PEC）两种方法（PEC-CUR 和 PEC-T-CUR）制备的，并应用于 HeLa、HT29 和 PC3 癌细胞的凋亡。PEC-T-CUR（使用超声匀浆器混合的离子液体（IL））的结果明显优于 PEC-CUR（使用磁力搅拌混合的 IL），对 HeLa 癌细胞的 IC 提高了 5.13 倍（从 1675.2 到 326.7μg/mL）。通过该方法制备的 PEC 具有良好的特性，如粒径、疏水性、pH 溶胀性。除此之外，两种情况下 IL 都可以定量回收。CUR 的包封水平高达 100%左右。溶胀、溶解/降解和 pH 值测定表明，PEC 可能在多种环境中发挥积极作用，释放 CUR、CHT 和 CS。通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、热重分析（TGA）、差示扫描量热法（DSC）和广角 X 射线散射（WAXS）的表征证实了两种 PEC 中均存在 CUR，细胞毒性研究表明含有 CUR 的 PEC 具有显著的抗癌作用。
Zotero 插件