Tonoyan Lilit, Guihéneuf Freddy, Friel Ruari, O'Flaherty Vincent
Westway Health, National University of Ireland Galway, Galway, Ireland.
Inalve, Nice, France.
Bio Protoc. 2020 Nov 5;10(21):e3813. doi: 10.21769/BioProtoc.3813.
This protocol details the construction of a simple, low-cost, small-scale, multiplex chemostat system designed for the continuous cultivation of microorganisms in suspension (, bacteria, yeast, microalgae). The continuous culture device can operate at a working volume of 25 ml and allows the run of 8 chemostats in parallel by a single person. It provides a platform for parallel, long-term studies of evolution and adaptation of microorganisms under the stress of antimicrobial agents and/or toxic pollutants. The system complies with the varied needs of researchers for an accessible, highly-throughput and reliable tool that is nevertheless easy to construct, use and operate, and not demanding of space, materials, medium supply and workload. Here, we also validate the use of this system to generate resistance towards a novel antimicrobial and a commonly used antibiotic in an antimicrobial-sensitive model organism. We believe that this "Do It Yourself" (DIY) system may constitute a useful tool to address the global problem of antibiotic resistance and to develop non-antibiotic based therapies.
本方案详细介绍了一种简单、低成本、小规模的多重恒化器系统的构建，该系统专为悬浮状态下的微生物（如细菌、酵母、微藻）连续培养而设计。该连续培养装置工作体积为25毫升，单人即可并行操作8个恒化器。它为在抗菌剂和/或有毒污染物压力下微生物的进化和适应性进行平行、长期研究提供了一个平台。该系统满足了研究人员对一种易于构建、使用和操作，且对空间、材料、培养基供应和工作量要求不高的、可获取的、高通量且可靠的工具的各种需求。在此，我们还验证了该系统在对抗菌敏感的模式生物中产生对新型抗菌剂和常用抗生素抗性方面的用途。我们相信，这种“自己动手做”（DIY）系统可能成为解决全球抗生素耐药性问题和开发非抗生素疗法的有用工具。
