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黏液虫寄生虫从头转录组组装数据。

Data of de novo transcriptome assembly of the myxozoan parasite .

作者信息

Kumar Gokhlesh, Ertl Reinhard, Nilsen Frank, Bartholomew Jerri L, El-Matbouli Mansour

机构信息

Clinical Division of Fish Medicine, University of Veterinary Medicine Vienna, Vienna, Austria.

VetCore Facility, University of Veterinary Medicine Vienna, Vienna, Austria.

出版信息

Data Brief. 2021 Feb 4;35:106831. doi: 10.1016/j.dib.2021.106831. eCollection 2021 Apr.

DOI:10.1016/j.dib.2021.106831
PMID:33659593
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7890133/
Abstract

a myxozoan endoparasite, causes proliferative kidney disease in salmonids. The life cycle of occurs between invertebrate bryozoan and vertebrate fish hosts. develops in the body cavity of colonial bryozoan and spores are released from mature spore sacs into the water likely through the vestibular pore and infect fish by attaching to their gills. However, very little is known about the transcriptome of this important parasite, which hampers studies into the molecular mechanisms of host-parasite interactions and understanding the parasite biology. In order to circumvent this limitation, we performed transcriptome assembly on the sacs of , collected from infected bryozoan . A total of 111.5 million filtered paired-end reads was obtained and assembled into 25,908 contigs corresponding to putative transcripts that were functionally annotated. More than 50% of the assembled transcripts (13,071 contigs) had a significant hit in NCBI non-redundant database. Based on Gene ontology annotation, the most highly scored categories of molecular function of the contigs were related to binding and catalytic activities in . This study provides a global overview of the transcriptome that will be a valuable resource for identifying virulence factors, gene discovery, genome annotation, and vaccine development applications. This data is accessible via NCBI BioProject (PRJNA680464).

摘要

一种粘孢子虫内寄生虫,可导致鲑科鱼类发生增殖性肾病。其生命周期发生在无脊椎动物苔藓虫和脊椎动物鱼类宿主之间。它在群体苔藓虫的体腔内发育,孢子可能通过前庭孔从成熟的孢子囊中释放到水中,并通过附着在鱼鳃上感染鱼类。然而,对于这种重要寄生虫的转录组了解甚少,这阻碍了对宿主 - 寄生虫相互作用分子机制的研究以及对寄生虫生物学的理解。为了克服这一限制,我们对从受感染的苔藓虫中收集的孢子囊进行了转录组组装。共获得1.115亿条过滤后的双端读数,并组装成25,908个重叠群,对应于经过功能注释的假定转录本。超过50%的组装转录本(13,071个重叠群)在NCBI非冗余数据库中有显著匹配。基于基因本体注释,重叠群分子功能得分最高的类别与结合和催化活性有关。本研究提供了该寄生虫转录组的全局概述,这将是鉴定毒力因子、基因发现、基因组注释和疫苗开发应用的宝贵资源。该数据可通过NCBI生物项目(PRJNA680464)获取。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe63/7890133/2257d05309d4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe63/7890133/726cea3b6458/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe63/7890133/1fc385f70753/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe63/7890133/2257d05309d4/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe63/7890133/726cea3b6458/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe63/7890133/1fc385f70753/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fe63/7890133/2257d05309d4/gr3.jpg

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Measurement of mRNA abundance using RNA-seq data: RPKM measure is inconsistent among samples.
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Sci Rep. 2023 May 17;13(1):8015. doi: 10.1038/s41598-023-34248-y.
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