Chen Shuai, Li Ruoyu, Ma Yaying, Lei Siru, Ming Ray, Zhang Xingtan
College of Life Science, Center for Genomics and Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, China.
Mitochondrial DNA B Resour. 2021 Feb 8;6(2):395-396. doi: 10.1080/23802359.2020.1869615.
cultivar Tieguanyin (TGY) is an important Oolong tea variety in China. In this study, we reported a complete chloroplast (cp) genome based on the Illumina sequencing technology and combined and reference-guided assembly strategies. The complete cp genome of 'TGY' displayed the regular quadripartite structure: a total of 157,126 bp in length, comprising a large single-copy (LSC, 86,904 bp) region, a small single-copy (SSC, 18,532 bp) region, and a pair of inverted repeats (IRs, 26,095 bp) regions. A lot of 132 predicted genes, including 87 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The overall GC content is 37.3%. Maximum likelihood (ML) phylogenetic tree involving 18 cp genomes of the genus revealed a relatively independent event of local domestication among three types of cultivars. The complete cp genome of 'TGY' provides an insight into tea plants for further understanding evolutionary research on tea plants.
品种铁观音(TGY)是中国重要的乌龙茶品种。在本研究中,我们基于Illumina测序技术以及组合和参考引导组装策略报道了一个完整的叶绿体(cp)基因组。“TGY”的完整cp基因组呈现出规则的四分体结构:全长157,126 bp,包括一个大单拷贝(LSC,86,904 bp)区域、一个小单拷贝(SSC,18,532 bp)区域和一对反向重复(IRs,26,095 bp)区域。共预测到132个基因,包括87个蛋白质编码基因、37个tRNA基因和8个rRNA基因。总体GC含量为37.3%。涉及该属18个cp基因组的最大似然(ML)系统发育树揭示了三种类型品种之间相对独立的局部驯化事件。“TGY”的完整cp基因组为深入了解茶树提供了帮助,以便进一步开展茶树进化研究。