Mosier Jenna A, Wu Yusheng, Reinhart-King Cynthia A
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
Fac Rev. 2021 Jan 28;10:8. doi: 10.12703/r/10-8. eCollection 2021.
Migration is an energy-intensive, multi-step process involving cell adhesion, protrusion, and detachment. Each of these steps require cells to generate and consume energy, regulating their morphological changes and force generation. Given the need for energy to move, cellular metabolism has emerged as a critical regulator of both single cell and collective migration. Recently, metabolic heterogeneity has been highlighted as a potential determinant of collective cell behavior, as individual cells may play distinct roles in collective migration. Several tools and techniques have been developed and adapted to study cellular energetics during migration including live-cell probes to characterize energy utilization and metabolic state and methodologies to sort cells based on their metabolic profile. Here, we review the recent advances in techniques, parsing the metabolic heterogeneities inherent in cell populations and their contributions to cell migration.
迁移是一个能量密集型的多步骤过程，涉及细胞黏附、突起和脱离。这些步骤中的每一个都需要细胞产生和消耗能量，以调节其形态变化和力的产生。鉴于迁移需要能量，细胞代谢已成为单细胞迁移和集体迁移的关键调节因子。最近，代谢异质性已被强调为集体细胞行为的一个潜在决定因素，因为单个细胞可能在集体迁移中发挥不同的作用。已经开发并采用了几种工具和技术来研究迁移过程中的细胞能量学，包括用于表征能量利用和代谢状态的活细胞探针，以及根据细胞代谢谱对细胞进行分选的方法。在这里，我们回顾了技术方面的最新进展，剖析了细胞群体中固有的代谢异质性及其对细胞迁移的贡献。
