Institut Pasteur, Mechanisms of Epigenetic Inheritance, Department of Developmental and Stem Cell Biology, UMR3738, CNRS, 75724, Paris, France.
Sorbonne Université, Collège Doctoral, F-75005, Paris, France.
Nat Commun. 2021 Mar 4;12(1):1441. doi: 10.1038/s41467-021-21691-6.
Inheritance and clearance of maternal mRNAs are two of the most critical events required for animal early embryonic development. However, the mechanisms regulating this process are still largely unknown. Here, we show that together with maternal mRNAs, C. elegans embryos inherit a complementary pool of small non-coding RNAs that facilitate the cleavage and removal of hundreds of maternal mRNAs. These antisense small RNAs are loaded into the maternal catalytically-active Argonaute CSR-1 and cleave complementary mRNAs no longer engaged in translation in somatic blastomeres. Induced depletion of CSR-1 specifically during embryonic development leads to embryonic lethality in a slicer-dependent manner and impairs the degradation of CSR-1 embryonic mRNA targets. Given the conservation of Argonaute catalytic activity, we propose that a similar mechanism operates to clear maternal mRNAs during the maternal-to-zygotic transition across species.
母体 mRNA 的遗传和清除是动物早期胚胎发育所必需的两个最重要的事件。然而，调节这一过程的机制在很大程度上仍然未知。在这里，我们表明，与母体 mRNAs 一起，秀丽隐杆线虫胚胎还继承了一组互补的小非编码 RNA，这些 RNA 有助于切割和去除数百种母体 mRNAs。这些反义小 RNA 被装载到母体具有催化活性的 Argonaute CSR-1 中，并切割不再参与体细胞分裂球翻译的互补 mRNA。在胚胎发育过程中特异性诱导 CSR-1 的消耗会导致依赖于 slicer 的胚胎致死，并损害 CSR-1 胚胎 mRNA 靶标的降解。鉴于 Argonaute 催化活性的保守性，我们提出在跨物种的母源到合子过渡过程中，可能存在一种类似的机制来清除母体 mRNAs。
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