Systems Biology of Gene Regulatory Elements, Max Delbrück Center Berlin, Berlin, Germany.
Systems Biology of Gene Regulatory Elements, Max Delbrück Center Berlin, Berlin, Germany
EMBO J. 2014 Aug 18;33(16):1740-50. doi: 10.15252/embj.201488117. Epub 2014 Jun 3.
Development of the early embryo is thought to be mainly driven by maternal gene products and post-transcriptional gene regulation. Here, we used metabolic labeling to show that RNA can be transferred by sperm into the oocyte upon fertilization. To identify genes with paternal expression in the embryo, we performed crosses of males and females from divergent Caenorhabditis elegans strains. RNA sequencing of mRNAs and small RNAs in the 1-cell hybrid embryo revealed that about one hundred sixty paternal mRNAs are reproducibly expressed in the embryo and that about half of all assayed endogenous siRNAs and piRNAs are also of paternal origin. Together, our results suggest an unexplored paternal contribution to early development.
早期胚胎的发育被认为主要是由母源基因产物和转录后基因调控驱动的。在这里，我们使用代谢标记显示，精子可以在受精时将 RNA 转移到卵母细胞中。为了鉴定在胚胎中有父本表达的基因，我们进行了来自不同的秀丽隐杆线虫品系的雄性和雌性之间的杂交。在 1 细胞杂种胚胎中，mRNA 和小 RNA 的 RNA 测序显示，大约 160 个父本 mRNA 可在胚胎中重复表达，并且大约一半的所有测定的内源性 siRNA 和 piRNA 也来自父本。总之，我们的结果表明，早期发育存在一种尚未被探索的父本贡献。
