Bergqvist Y, Hed K, Karlberg B
Department of Clinical Chemistry, Falun Central Hospital, Sweden.
Int J Sports Med. 1988 Feb;9(1):73-6. doi: 10.1055/s-2007-1024982.
An improved enzymatic fluorometric method for automated analysis of lactate in perchloric acid extracts of capillary blood with flow injection (FIA) is described. The concentrations of lactate can be measured in as little as 20 microliter capillary blood from an exercising person. Within-day and between-day coefficients of variation are about 2%. The recovery of lactate from whole blood is 101%. Lactate is stable in perchloric acid extracts for at least 15 days at room temperature, and at +4 degrees C at least 30 days. Blank fluorescence in perchloric acid extracts of blood is low with the improved method. The FIA system is simple to use, inexpensive to operate, and is recommended for any laboratory with a high throughput of samples.
描述了一种改进的酶促荧光法，用于通过流动注射（FIA）对毛细血管血的高氯酸提取物中的乳酸进行自动分析。可以从运动者的仅20微升毛细血管血中测量乳酸浓度。日内和日间变异系数约为2%。全血中乳酸的回收率为101%。乳酸在高氯酸提取物中于室温下至少稳定15天，在4℃下至少稳定30天。采用改进方法时，血液高氯酸提取物中的空白荧光较低。该流动注射分析系统使用简单，操作成本低，推荐给任何样本通量高的实验室。
