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血液中丙酮酸和乳酸测定的自动酶促荧光法。

Automated enzymatic fluorometric method for the determination of pyruvic and lactic acids in blood.

作者信息

Cramp D G

出版信息

J Clin Pathol. 1968 Mar;21(2):171-4. doi: 10.1136/jcp.21.2.171.

Abstract

A simple automated fluorometric method for the estimation of lactate and pyruvate is described. Analyses can be performed at 60/hour and can conveniently determine pyruvate and lactate in the ranges 0-5 mg. and 0-30 mg/100 ml. The methods give a high degree of analytical precision and good yields from recovery experiments.

摘要

本文描述了一种用于估算乳酸和丙酮酸的简单自动荧光法。每小时可进行60次分析,能够方便地测定0 - 5毫克和0 - 30毫克/100毫升范围内的丙酮酸和乳酸。这些方法具有高度的分析精度,回收实验的回收率良好。

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引用本文的文献

本文引用的文献

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AUTOMATED LACTIC ACID DETERMINATION IN SERUM AND TISSUE EXTRACTS.
Anal Biochem. 1965 Feb;10:304-17. doi: 10.1016/0003-2697(65)90271-x.
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Blood pyruvate concentration measured by a specific method in control subjects.
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Observations on an enzymic method for the estimation of pyruvate in blood.
Clin Chim Acta. 1962 Mar;7:206-11. doi: 10.1016/0009-8981(62)90011-6.
5
The fluorometric measurement of pyridine nucleotides.
J Biol Chem. 1957 Feb;224(2):1047-64.
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Control of concentration gradients of pyruvate and lactate across cell membranes in blood.
J Appl Physiol. 1956 Sep;9(2):163-70. doi: 10.1152/jappl.1956.9.2.163.
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