Sharma Madhulika, Yerrathota Sireesha, Thornton Mackenzie M, Gunewardena Sumedha
Departments of Internal Medicine, United States.
The Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, KS, United States.
Data Brief. 2021 Feb 13;35:106873. doi: 10.1016/j.dib.2021.106873. eCollection 2021 Apr.
The Notch signaling pathway is an important conserved pathway for normal homeostasis during development. However, targeted deletion of Notch4 ( ) or Notch3 ( ) in mice is not lethal. In fact, both and mice develop normally and are fertile. Here we present RNA seq analysis of differential gene expression in the kidneys of mice versus the mice, all on FVB background. Kidneys were collected from and littermates at 3 months of age. RNA sequencing was carried out. The raw data were analyzed for differential gene expression using a negative binomial generalized linear model in the DeSeq2 software package. We used -value ≤0.05 and an absolute fold change of 1.5 or greater to identify top upregulated and downregulated genes in mice compared to mice. The data provided will indentify targets of Notch3 and Notch4 signaling, specifically in kidney diseases where Notch3 or Notch4 are abberantly or redundantly expressed.
Notch信号通路是发育过程中正常稳态的重要保守通路。然而，在小鼠中靶向缺失Notch4（ ）或Notch3（ ）并非致命。事实上， 和 小鼠均正常发育且可育。在此，我们展示了在FVB背景下， 小鼠与 小鼠肾脏中差异基因表达的RNA测序分析。在3月龄时从 和 同窝小鼠中收集肾脏。进行RNA测序。使用DeSeq2软件包中的负二项广义线性模型对原始数据进行差异基因表达分析。我们使用≤0.05的P值和1.5或更大的绝对倍数变化来鉴定与 小鼠相比， 小鼠中上调和下调的顶级基因。所提供的数据将确定Notch3和Notch4信号的靶点，特别是在Notch3或Notch4异常或冗余表达的肾脏疾病中。
