Simrén Joel, Ashton Nicholas J, Blennow Kaj, Zetterberg Henrik
Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy University of Gothenburg Mölndal Sweden.
Clinical Neurochemistry Laboratory Sahlgrenska University Hospital Mölndal Sweden.
Alzheimers Dement (Amst). 2021 Feb 20;13(1):e12145. doi: 10.1002/dad2.12145. eCollection 2021.
This study investigated alternative pre-analytical handling of blood for neurofilament light (NfL) analysis where resources are limited.
Plasma NfL was measured with single molecule array after alternative blood processing procedures: dried plasma spots (DPS), dried blood spots (DBS), and delayed 48-hour centrifugation. These were compared to standardized plasma processing (reference standard [RS]). In a discovery cohort (n = 10) and a confirmatory cohort (n = 21), whole blood was obtained from individuals with unknown clinical etiology. In the confirmatory cohort, delayed centrifugation protocol was paired with either 37°C incubation or sample shaking to test the effect of these parameters.
Delayed centrifugation (R = 0.991) and DPS (discovery cohort, R= 0.954; confirmatory cohort, DPS: R = 0.961) methods were strongly associated with the RS. Delayed centrifugation with higher temperatures (R = 0.995) and shaking (R = 0.975) did not affect this association. DPS (< 0.001) returned concentrations considerably lower than the RS.
DPS or delayed centrifugation are viable pre-analytical procedures for the accurate quantification of plasma NfL.
本研究调查了在资源有限的情况下，用于神经丝轻链（NfL）分析的血液的替代分析前处理方法。
在采用替代血液处理程序后，用单分子阵列测量血浆NfL：干血浆斑（DPS）、干血斑（DBS）和延迟48小时离心。将这些方法与标准化血浆处理（参考标准[RS]）进行比较。在一个发现队列（n = 10）和一个验证队列（n = 21）中，从临床病因不明的个体获取全血。在验证队列中，将延迟离心方案与37°C孵育或样品振荡配对，以测试这些参数的影响。
延迟离心（R = 0.991）和DPS（发现队列，R = 0.954；验证队列，DPS：R = 0.961）方法与RS密切相关。较高温度下的延迟离心（R = 0.995）和振荡（R = 0.975）并不影响这种相关性。DPS（< 0.001）得出的浓度明显低于RS。
DPS或延迟离心是准确定量血浆NfL的可行分析前程序。
