Ministry of Education, Nankai University, School of Physics, Key Laboratory of Weak-Light Nonlinear, China.
J Biomed Opt. 2021 Mar;26(3). doi: 10.1117/1.JBO.26.3.036501.
Large space-bandwidth product is highly desirable in many biomedical imaging. Fourier ptychographic microscopy (FPM) is a computational imaging technique that can significantly increase the space-bandwidth product of a standard microscope. The illuminator of a Fourier ptychographic microscope is not flexible at present, and it is inconvenient to meet different imaging needs.
An illuminator based on a two-axis motorized rotation stage was presented to provide a more flexible illuminating way with the goal of meeting different imaging needs.
The illuminator adopts a concentric illuminating method to provide coherent illumination in any direction on the sample plane. The sampling pattern can be freely designed and changed according to the parameters of the imaging system. A dither removing algorithm was proposed to remove the potential dither influence introduced in the image acquisition process.
The illuminator could be conveniently integrated into different imaging systems. The feasibility and flexibility were demonstrated by applying it to imaging systems with numerical aperture of 0.045 and 0.01. The resolution gain is about 4- and 13-fold, respectively. The effectiveness of the dither removing algorithm was validated in both simulation and experiment.
A more flexible illuminator for FPM was presented to meet different imaging needs. A dither removing algorithm was proposed to remove dither influence.
在许多生物医学成像中，大的空间带宽积是非常理想的。傅里叶叠层显微镜（FPM）是一种计算成像技术，可以显著提高标准显微镜的空间带宽积。目前，傅里叶叠层显微镜的照明器不灵活，不方便满足不同的成像需求。
提出了一种基于两轴电机旋转台的照明器，提供了一种更灵活的照明方式，以满足不同的成像需求。
该照明器采用同心照明方法，可在样品平面上的任何方向提供相干照明。采样图案可根据成像系统的参数自由设计和更改。提出了一种去抖动算法，以去除图像采集过程中可能引入的抖动影响。
该照明器可以方便地集成到不同的成像系统中。通过将其应用于数值孔径分别为 0.045 和 0.01 的成像系统，验证了其可行性和灵活性。分辨率增益分别约为 4 倍和 13 倍。抖动去除算法在模拟和实验中均得到了验证。
提出了一种更灵活的 FPM 照明器，以满足不同的成像需求。提出了一种去抖动算法，以去除抖动影响。
