Line S R, Torloni H, Montes G S, Junqueira L C
Department of Pathology, Dental School, University of Campinas, São Paulo, Brazil.
Histochemistry. 1988;88(3-6):411-3. doi: 10.1007/BF00570303.
Using only one histologic preparation and under the light microscope, the simple Picrosirius-polarization method permitted the histochemical characterization of the collagenous nature of amianthoid fibers in file cases of salivary gland tumors. In this regard the foregoing results agree with the electron microscopic and X-ray diffraction observations recorded in the literature. Not only did the Picrosirius-polarization method permit the precise characterization of the collagenous nature of asbestoid change but it was also useful for studying the degree of collagen polymerization in the lesion. Collagen molecules in the amianthoid fibers showed hyperpolymerization whereas the molecules in the compact areas were disoriented. Since the foregoing results demonstrate that the Picrosirius-polarization method is a simple and sensitive procedure for detecting asbestoid change in cartilage sections obtained from paraffin-embedded tissues, the usefulness of this technique for studying file cases is evident.
仅使用一种组织学标本并在光学显微镜下，简单的天狼星红偏振法就能对唾液腺肿瘤存档病例中的石棉样纤维的胶原性质进行组织化学表征。在这方面，上述结果与文献中记录的电子显微镜和X射线衍射观察结果一致。天狼星红偏振法不仅能精确表征石棉样改变的胶原性质，还可用于研究病变中胶原聚合程度。石棉样纤维中的胶原分子呈现高度聚合，而致密区域的分子则排列紊乱。鉴于上述结果表明天狼星红偏振法是检测石蜡包埋组织软骨切片中石棉样改变的一种简单且灵敏的方法，该技术在研究存档病例中的实用性显而易见。
