Liu Mengyuan, Tang Lizhu, Hu Chenyan, Sun Baili, Huang Zileng, Chen Lianguo
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; University of Chinese Academy of Sciences, Beijing 100049, China.
School of Chemistry and Environmental Engineering, Wuhan Institute of Technology, Wuhan 430072, China.
Ecotoxicol Environ Saf. 2021 May;214:112107. doi: 10.1016/j.ecoenv.2021.112107. Epub 2021 Mar 2.
Perfluorobutanesulfonate (PFBS) pollutant and probiotic bacteria can interact to affect the reproductive outcomes of zebrafish. However, it is still unexplored how the growth and health of offspring are modulated by the combination of PFBS and probiotic. In the present study, adult zebrafish were exposed to 0 and 10 μg/L PFBS for 40 days, with or without dietary supplementation of probiotic Lactobacillus rhamnosus. After parental exposure, the development, growth and viability of offspring larvae were examined, with the integration of molecular clues across proteome fingerprint, growth hormone/insulin-like growth factor (GH/IGF) axis, calcium homeostasis, hypothalamic-pituitary-adrenal (HPA) axis and nutrient metabolism. Parental probiotic supplementation significantly increased the body weight and body length of offspring larvae. Despite the spiking of PFBS, larvae from the combined exposure group still had longer body length. RNA processing and ribosomal assembly pathways may underlie the enhancement of offspring growth by probiotic bacteria. However, the presence of PFBS remarkably increased the concentrations of cortisol hormone in offspring larvae as means to cope with the xenobiotic stress, which required more energy production. As evidenced by the proteomic analysis, the addition of probiotic bacteria likely alleviated the energy metabolism disorders of PFBS, thus allocating more energy for the larval offspring growth from the combined group. It was noteworthy that multiple molecular disturbances caused by PFBS were antagonized by probiotic additive. Overall, the present study elucidated the intergenerational interaction between PFBS and probiotic on offspring growth and health after parental exposure.
全氟丁烷磺酸（PFBS）污染物与益生菌可相互作用，影响斑马鱼的繁殖结果。然而，PFBS与益生菌的组合如何调节后代的生长和健康仍未得到探索。在本研究中，成年斑马鱼暴露于0和10μg/L的PFBS中40天，同时给予或不给予膳食补充益生菌鼠李糖乳杆菌。亲代暴露后，对后代幼虫的发育、生长和活力进行了检查，并整合了蛋白质组指纹图谱、生长激素/胰岛素样生长因子（GH/IGF）轴、钙稳态、下丘脑-垂体-肾上腺（HPA）轴和营养代谢等方面的分子线索。亲代补充益生菌显著增加了后代幼虫的体重和体长。尽管PFBS浓度升高，但联合暴露组的幼虫体长仍然较长。RNA加工和核糖体组装途径可能是益生菌促进后代生长的基础。然而，PFBS的存在显著增加了后代幼虫中皮质醇激素的浓度，作为应对外源性应激的手段，这需要更多的能量产生。蛋白质组学分析表明，添加益生菌可能缓解了PFBS引起的能量代谢紊乱，从而为联合组的幼虫后代生长分配了更多能量。值得注意的是，PFBS引起的多种分子干扰被益生菌添加剂所拮抗。总体而言，本研究阐明了亲代暴露后PFBS与益生菌对后代生长和健康的代际相互作用。
