抗菌光动力疗法用母液及其单一化合物作为光敏剂。

Antimicrobial Photodynamic Treatment with Mother Juices and Their Single Compounds as Photosensitizers.

机构信息

Institute of Forensic Medicine, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.

Department of Operative Dentistry and Periodontology, Medical Center, Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.

出版信息

Nutrients. 2021 Feb 24;13(3):710. doi: 10.3390/nu13030710.

The potent antimicrobial effects of antimicrobial photodynamic therapy (aPDT) with visible light plus water-filtered infrared-A irradiation and natural compounds as photosensitizers (PSs) have recently been demonstrated. The aim of this study was to obtain information on the antimicrobial effects of aPDT with mother juices against typical cariogenic oral pathogens in their planktonic form and determine its eradication potential on total human salivary bacteria from volunteers. Mother juices of pomegranate, bilberry, and chokeberry at different concentrations were used as PSs. The unweighted (absolute) irradiance was 200 mW cm, applied five minutes. Planktonic cultures of and and total mixed bacteria from pooled saliva of volunteers were treated with aPDT. Up to more than 5 log of and were killed by aPDT with 0.4% and 0.8% pomegranate juice, 3% and 50% chokeberry juice, and 12.5% bilberry juice (both strains). Concentrations of at least 25% (pomegranate) and >50% (chokeberry and bilberry) eradicated the mixed bacteria in saliva samples. This pilot study has shown that pomegranate mother juice is superior to the berry juices as a multicomponent PS for killing pathogenic oral bacteria with aPDT.

可见光源联合水滤红外线 A 辐射和天然化合物作为光敏剂的光动力抗菌疗法（aPDT）具有强大的抗菌效果，这一点最近已得到证实。本研究的目的是获取关于母体汁液的 aPDT 对浮游形式的典型致龋口腔病原体的抗菌效果的信息，并确定其对志愿者总唾液细菌的根除潜力。石榴、越桔和黑果腺肋花楸的不同浓度母体汁液被用作 PS。未加权（绝对）辐照度为 200 mW cm，应用 5 分钟。浮游培养的和以及志愿者混合唾液中的总混合细菌用 aPDT 处理。用 0.4%和 0.8%石榴汁、3%和 50%黑果腺肋花楸汁以及 12.5%越桔汁（两种菌株）处理后，可减少 5 个对数以上。浓度至少为 25%（石榴）和>50%（黑果腺肋花楸和越桔）可消除唾液样本中的混合细菌。这项初步研究表明，与浆果汁相比，石榴母体汁液作为一种多成分 PS，更适合用于杀灭致龋口腔细菌的 aPDT。