Satoh Yutaroh, Ishihara Kazuyuki
Faculty of Dentistry, Tokyo Dental College, 2-9-18, Kandamisaki-cho, Chiyoda-ku, Tokyo, Japan.
Department of Microbiology, Tokyo Dental College, 2-9-18, Kandamisaki-cho, Chiyoda-ku, Tokyo, Japan.
J Oral Biosci. 2020 Jun;62(2):169-174. doi: 10.1016/j.job.2020.01.009. Epub 2020 Feb 11.
The purpose of this study was to isolate the active antibacterial compounds from Bilberry (Vaccinium myrtillus L.) against periodontopathic bacteria.
The acetone soluble fraction of Bilberry was extracted from the oil layer by oil/water separation. The extract was then purified by a silica gel open column chromatography. The minimum inhibitory concentration (MIC) of the total extract or purified fractions against bacteria was measured at each step.
The MIC of the total extract against Porphyromonas gingivalis was 500 μg/mL. The fraction exhibiting antibacterial activity against P. gingivalis was called NU4-TDC, and its MICs against P. gingivalis, Fusobacterium nucleatum, and Prevotella intermedia were 26.0 ± 7.8 μg/mL, 59.0 ± 10.4 μg/mL, and 45.1 ± 16.5 μg/mL, respectively. The MIC against Streptococcus mutans was >62.5 μg/mL.
Bilberry contains antibacterial components against periodontopathic bacteria, such as P. gingivalis, F. nucleatum, and P. intermedia.
本研究旨在从越橘（欧洲越橘）中分离出对牙周病原菌具有活性的抗菌化合物。
通过油/水分离从油层中提取越橘的丙酮可溶部分。然后通过硅胶开放柱色谱法对提取物进行纯化。在每个步骤中测量总提取物或纯化部分对细菌的最低抑菌浓度（MIC）。
总提取物对牙龈卟啉单胞菌的MIC为500μg/mL。对牙龈卟啉单胞菌表现出抗菌活性的部分称为NU4-TDC，其对牙龈卟啉单胞菌、具核梭杆菌和中间普氏菌的MIC分别为26.0±7.8μg/mL、59.0±10.4μg/mL和45.1±16.5μg/mL。对变形链球菌的MIC>62.5μg/mL。
越橘含有针对牙周病原菌的抗菌成分，如牙龈卟啉单胞菌、具核梭杆菌和中间普氏菌。
