Kasambala Donga Trust, Meadow Richard, Meyling Nicolai V, Klingen Ingeborg
Bunda College Campus, Crop and Soil Sciences Department, Lilongwe University of Agriculture and Natural Resources (LUANAR), P.O. Box 219 Lilongwe, Malawi.
Department of Plant Sciences, Faculty of Biosciences, Norwegian University of Life Sciences (NMBU), P.O. Box 5003, 1432 Ås, Norway.
Insects. 2021 Feb 13;12(2):160. doi: 10.3390/insects12020160.
The natural occurrence of entomopathogenic fungal endophytes in sugarcane () and in soil samples from sugarcane fields was evaluated in Chikwawa District, southern Malawi. Fungi from soil were isolated by baiting using larva. Fungal endophytes were isolated from surface-sterilized plant tissue sections. Forty-seven isolates resembled the genus , 9 isolates were , and 20 isolates were . There was no significant difference in the number and type of fungal isolates collected from soil and from plant tissue. There was, however, a significant difference in the part of the plant where fungal species were isolated, which fungal species were isolated, and the number of fungal species isolated at each location. Phylogenetic analysis of 47 isolates based on DNA sequencing of the Bloc intergenic region indicated that these isolates all belonged to and aligned with sequences of isolates of African and Neotropical origin. The Malawian isolates formed a distinct clade. No larvae died from infestation by multiple fungi. To the best of our knowledge, this is the first report of and spp. occurring naturally as endophytes in sugarcane. Further, it is the first report of , spp., and spp. in the soil of sugarcane fields in Africa.
在马拉维南部的奇夸瓦区，对甘蔗（）以及甘蔗田土壤样本中昆虫病原真菌内生菌的自然发生情况进行了评估。通过用幼虫诱饵法从土壤中分离真菌。从表面消毒的植物组织切片中分离真菌内生菌。47个分离株类似于属，9个分离株是，20个分离株是。从土壤和植物组织中收集的真菌分离株的数量和类型没有显著差异。然而，在分离真菌物种的植物部位、分离出的真菌物种以及每个地点分离出的真菌物种数量方面存在显著差异。基于Bloc基因间区域DNA测序对47个分离株进行的系统发育分析表明，这些分离株均属于，并且与非洲和新热带起源的分离株序列一致。马拉维的分离株形成了一个独特的进化枝。没有幼虫因多种真菌感染而死亡。据我们所知，这是关于和 spp. 作为甘蔗内生菌自然发生的首次报告。此外，这是非洲甘蔗田土壤中、 spp. 和 spp. 的首次报告。
