Department of Plant Protection, Faculty of Agriculture, Tishreen University, Latakia, Syria.
Laboratory of Mycology, Department of Plant Protection, Lebanese Agricultural Research Institute, Fanar, Lebanon.
J Invertebr Pathol. 2023 Feb;196:107868. doi: 10.1016/j.jip.2022.107868. Epub 2022 Nov 28.
A survey was conducted to investigate endophytic Beauveria spp. and associated fungi in the tissues of cucumber plants (Cucumis sativus L.) cultivated in open fields and greenhouses in some regions of Syria during 2018-2019. Cultures of fungal endophytes belonging to nine genera were obtained (frequency %): Aspergillus (87.1%), Penicillium (41.23%), Fusarium (38.15%), Beauveria (12.83%), Trichoderma (9.87%), Colletotrichum (4.36%), Cladosporium (3.54%), Alternaria (2.79%), and Chaetomium (2.2%). Only Beauveria and Trichoderma cultures were entomopathogenic. Beauveria bassiana isolates were identified morphologically and molecularly from the stems, leaves, petioles, and fruits of cucumber plants collected at seven agricultural sites. Their ability to artificially colonize all cucumber plant parts was confirmed in the laboratory using soil drench. In this study, the natural association between the endophytic entomopathogenic fungus B. bassiana and cucumber plants is newly reported. In addition, a checklist of plant species reported in the literature to harbor this fungus is also provided.
2018-2019 年，在叙利亚一些地区的露天和温室中种植的黄瓜植株（Cucumis sativus L.）组织内，对内生拟青霉属和相关真菌进行了调查。获得了属于九个属的真菌内生菌培养物（频率%）：曲霉属（87.1%）、青霉属（41.23%）、镰刀菌属（38.15%）、拟青霉属（12.83%）、木霉属（9.87%）、炭疽菌属（4.36%）、枝孢属（3.54%）、链格孢属（2.79%）和枝孢属（2.2%）。只有拟青霉属和木霉属的培养物具有昆虫病原性。从在七个农业地点采集的黄瓜植株的茎、叶、叶柄和果实中，通过形态学和分子学鉴定了内生昆虫病原真菌球孢白僵菌的分离株。在实验室中，通过土壤淋溶证实了它们能够人工定殖所有的黄瓜植物部分。在这项研究中，新报道了内生昆虫病原真菌球孢白僵菌与黄瓜植株之间的天然共生关系。此外，还提供了一份文献中报道的携带这种真菌的植物物种清单。
