用于……的深紫外发光二极管光疗法

Deep Ultraviolet Light-Emitting Diode Light Therapy for .

作者信息

Fukuda Soichiro, Ito Shunsuke, Nishikawa Jun, Takagi Tatsuya, Kubota Naoto, Otsuyama Ken-Ichiro, Tsuneoka Hidehiro, Nojima Junzo, Harada Koji, Mishima Katsuaki, Suehiro Yutaka, Yamasaki Takahiro, Sakaida Isao

机构信息

Department of Laboratory Science, Graduate School of Medicine, Yamaguchi University, Ube 7558505, Japan.

Department of Gastroenterology and Hepatology, Graduate School of Medicine, Yamaguchi University, Ube 7558505, Japan.

出版信息

Microorganisms. 2021 Feb 19;9(2):430. doi: 10.3390/microorganisms9020430.

BACKGROUND

, which is associated with periodontitis and gingivitis, has been detected in colorectal cancer (CRC).

METHODS

We evaluated the bactericidal effect of deep ultraviolet (DUV) light-emitting diode (LED) light therapy on both qualitatively and quantitatively. Two DUV-LEDs with peak wavelengths of 265 and 280-nm were used. DNA damage to was evaluated by the production of cyclobutane pyrimidine dimers (CPD) and pyrimidine (6-4) pyrimidone photoproducts (6-4PP).

RESULTS

DUV-LEDs showed a bactericidal effect on . No colony growth was observed after 3 min of either 265 nm or 280 nm DUV-LED irradiation. The survival rates of under 265 nm DUV-LED light irradiation dropped to 0.0014% for 10 s and to 0% for 20 s irradiation. Similarly, the survival rate of under 280 nm DUV-LED light irradiation dropped to 0.00044% for 10 s and 0% for 20 s irradiation. The irradiance at the distance of 35 mm from the DUV-LED was 0.265 mW/cm for the 265 nm LED and 0.415 mW/cm for the 280 nm LED. Thus, the radiant energy for lethality was 5.3 mJ/cm for the 265 nm LED and 8.3 mJ/cm for the 280 nm LED. Amounts of CPD and 6-4PP in irradiated with 265 nm DUV-LED light were 6.548 ng/µg and 1.333 ng/µg, respectively.

CONCLUSIONS

DUV-LED light exerted a bactericidal effect on by causing the formation of pyrimidine dimers indicative of DNA damage. Thus, DUV-LED light therapy may have the potential to prevent CRC.

背景

在结直肠癌（CRC）中已检测到与牙周炎和牙龈炎相关的[具体细菌名称未给出]。

方法

我们从定性和定量两方面评估了深紫外（DUV）发光二极管（LED）光疗法对[具体细菌名称未给出]的杀菌效果。使用了两个峰值波长分别为265和280纳米的DUV-LED。通过环丁烷嘧啶二聚体（CPD）和嘧啶（6-4）嘧啶酮光产物（6-4PP）的产生来评估对[具体细菌名称未给出]的DNA损伤。

结果

DUV-LED对[具体细菌名称未给出]显示出杀菌效果。在265纳米或280纳米DUV-LED照射3分钟后均未观察到菌落生长。在265纳米DUV-LED光照射下，[具体细菌名称未给出]照射10秒后的存活率降至0.0014%，照射20秒后降至0%。同样，在280纳米DUV-LED光照射下，[具体细菌名称未给出]照射10秒后的存活率降至0.00044%，照射20秒后降至0%。距离DUV-LED 35毫米处的辐照度，265纳米LED为0.265毫瓦/平方厘米，280纳米LED为0.415毫瓦/平方厘米。因此，265纳米LED的致死辐射能量为5.3毫焦/平方厘米，280纳米LED为8.3毫焦/平方厘米。用265纳米DUV-LED光照射的[具体细菌名称未给出]中CPD和6-4PP的量分别为6.548纳克/微克和1.333纳克/微克。