Department of Biomedical Sciences and Public Health, Università Politecnica Delle Marche, Ancona, Italy.
Department of Biomedical Sciences and Public Health, Università Politecnica Delle Marche, Ancona, Italy.
Nitric Oxide. 2021 Jan 1;106:66-71. doi: 10.1016/j.niox.2020.11.002. Epub 2020 Nov 10.
Nitric oxide (NO) produced in the oral cavity is a powerful resource for the human body, especially when NO-syntethase production is not adequate. The role of oral microbiome in determining blood pressure levels has been linked to the active role of some bacterial species involved in the nitro-reducing process. In the present study we investigated the correlation between selected oral microbiome characteristics, nitric oxide (NO) concentration in saliva and their association with hypertension.
A case-control study including 48 (25 normotensive and 23 hypertensive subjects), subjects between 50 and 70 years old, was carried out at the dental clinic of an Italian teaching hospital. Characteristics of participants have been evaluated by means of a physical examination, and by an assisted interview. A real-time polymerase chain reaction in samples of saliva and plaque was used to detect Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Tannerella forsythia, Porphyromonas gingivalis, Treponema denticola, Streptococcus mutans, Streptococcus sanguinis, Veillonella dispar and Neisseria subflava as well as total bacterial count. Nitric oxide in saliva was evaluated by the ELISA method.
Normotensive subjects, compared with hypertensive subjects, had significantly higher concentration of NO (165.77 ± 61.7 vs 57.49 ± 19.61 μmol/l; p = 0.023), and higher bacterial concentration of the supragingival plaque (4.73E+07 ± 4.33+07 vs 4.02E+07 ± 4.00+07; p = 0.024). Bacterial species, usually associated to good oral health status, such as Neisseria subflava, were significantly more present in normotensive subjects than in hypertensive ones (9090.88 ± 5481.49 vs 4791.35 ± 4349.37; p < 0.001). considering the concentration of bacteria as a biomarker of the development of hypertension.
The results support the association between hypertension, oral microbiome and salivary nitric oxide, in fact do the results allow us to establish any biomarkers (microbial or biochemical, NO) that allow early therapeutic intervention.
口腔中产生的一氧化氮（NO）是人体的强大资源，尤其是当 NO 合酶产生不足时。口腔微生物组在确定血压水平方面的作用与某些参与硝基还原过程的细菌物种的积极作用有关。在本研究中，我们调查了选定的口腔微生物组特征、唾液中的一氧化氮（NO）浓度及其与高血压之间的相关性。
这是一项包括 48 名（25 名血压正常和 23 名高血压患者）年龄在 50 至 70 岁之间的病例对照研究，在意大利一所教学医院的牙科诊所进行。通过体检和辅助访谈评估参与者的特征。实时聚合酶链反应用于检测唾液和牙菌斑样本中的伴放线放线杆菌、中间普氏菌、福赛斯拟杆菌、牙龈卟啉单胞菌、齿密螺旋体、变形链球菌、血链球菌、中间普氏菌和中间普氏菌以及总细菌计数。通过 ELISA 法评估唾液中的一氧化氮。
与高血压患者相比，血压正常的受试者唾液中一氧化氮浓度明显更高（165.77±61.7 与 57.49±19.61μmol/L；p=0.023），龈上菌斑中的细菌浓度也更高（4.73E+07±4.33+07 与 4.02E+07±4.00+07；p=0.024）。通常与良好口腔健康状态相关的细菌物种，如中间普氏菌，在血压正常的受试者中明显多于高血压患者（9090.88±5481.49 与 4791.35±4349.37；p<0.001）。将细菌浓度视为高血压发展的生物标志物。
研究结果支持高血压、口腔微生物组和唾液一氧化氮之间的关联，事实上，研究结果使我们能够确定任何生物标志物（微生物或生化、NO），以便进行早期治疗干预。
