Dias Aline Moreira, Borges Miguel, Blassioli Moraes Maria Carolina, Lorran Figueira Coelho Matheus, Čokl Andrej, Laumann Raúl Alberto
Zoology Post-Graduation Program, Institute of Biology, University of Brasilia, Brasília 70910-900, Brazil.
Semiochemicals Laboratory, Embrapa Genetic Resources and Biotechnology, Brasília 70770-917, Brazil.
Insects. 2021 Feb 18;12(2):177. doi: 10.3390/insects12020177.
Stink bugs are major pests in diverse crops around the world. Pest management strategies based on insect behavioral manipulation could help to develop biorational management strategies of stink bugs. Insect mating disruption using vibratory signals is an approach with high potential for pest management. The objective of this work was to investigate the effect of conspecific female rival signals on the mating behavior and copulation of three stink bug species to establish their potential for mating disruption. Previously recorded female rival signals were played back to bean plants where pairs of the Neotropical brown stink bug, , and two green stink bugs, and were placed. Vibratory communication and mating behavior were recorded for each pair throughout the experimental time (20 min). Female rival signals show a disrupting effect on the reproductive behavior of three conspecific investigated stink bug species. This effect was more clearly expressed in and than in . The likelihood of copulating in pairs placed on control plants, without rival signals, increased 29.41 times in , 4.6 times in and 1.71 times in . However, in the last case, the effect of female rivalry signals in copulation was not significant. The effect of mating disruption of female rival signals of the three stink bug species may originate from the observed reduction in specific vibratory communication signals emitted, which influences the duet formation and further development of different phases of mating behavior. Our results suggest that female rival signals have potential for application in manipulation and disruption of mating behavior of stink bugs. Further work needs to focus on the effects of female rival signals used in long duration experiments and also their interactions with chemical communication of stink bugs.
椿象是全球多种作物的主要害虫。基于昆虫行为操纵的害虫管理策略有助于制定椿象的生物合理管理策略。利用振动信号干扰昆虫交配是一种具有很大害虫管理潜力的方法。这项工作的目的是研究同种雌虫竞争信号对三种椿象交配行为和交尾的影响，以确定其干扰交配的潜力。将先前记录的雌虫竞争信号回放至种植有新热带褐椿象以及两种绿椿象（[此处原文缺失两种绿椿象的具体学名]）成对放置的菜豆植株上。在整个实验时间（20分钟）内记录每对椿象的振动通讯和交配行为。雌虫竞争信号对三种被研究的同种椿象的生殖行为具有干扰作用。这种作用在[此处原文缺失两种绿椿象的具体学名]中比在新热带褐椿象中表现得更明显。在没有竞争信号的对照植株上放置的成对椿象交尾的可能性，新热带褐椿象增加了29.41倍，[此处原文缺失一种绿椿象的具体学名]增加了4.6倍，[此处原文缺失另一种绿椿象的具体学名]增加了1.71倍。然而，在最后一种情况下，雌虫竞争信号对交尾的影响并不显著。三种椿象的雌虫竞争信号干扰交配的作用可能源于所观察到的特定振动通讯信号发射的减少，这影响了二重唱形成以及交配行为不同阶段的进一步发展。我们的结果表明，雌虫竞争信号在操纵和干扰椿象交配行为方面具有应用潜力。进一步的工作需要关注长时间实验中使用的雌虫竞争信号的影响以及它们与椿象化学通讯的相互作用。
