Program of Immunology & Microbiology, Department of Biomedicine & Health Sciences, Graduate School, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, Korea.
Department of Dermatology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, 222, Banpo-daero, Seocho-gu, Seoul 06591, Korea.
Int J Mol Sci. 2021 Feb 28;22(5):2418. doi: 10.3390/ijms22052418.
Human papillomavirus (HPV) in high-risk groups is known to suppress the type I interferon (IFN) signaling pathway leading to the transcription of interferon-stimulated genes (ISGs), which have many antiviral functions. However, the effects of HPV on the action of various ISGs in low-risk groups are not fully understood. We aimed to investigate whether antiviral ISGs are expressed in transfected keratinocytes with type 2 HPV (HPV-2) . The mRNA and protein expressions of ISGs and type I IFN signaling pathway components were evaluated by quantitative real-time polymerase chain reaction, western blot, immunofluorescence, and/or immunohistochemistry. Compared with normal skin, mRNA expression of all ISGs in HPV-2 positive cutaneous warts was significantly decreased ( < 0.05). In comparison with empty vector transfection, transfection significantly down-regulated the mRNA and protein expressions of ISGs and type I IFN signaling pathway components, which were significantly up-regulated by siRNA transfection ( < 0.05). Interestingly, epigallocatechin-3-gallate (EGCG) pretreatment up-regulated the mRNA and protein expressions of ISGs and type I IFN signaling pathway components, which were significantly down-regulated by transfection ( < 0.05). Our results demonstrate that EGCG is a potential candidate for cutaneous wart prevention.
人乳头瘤病毒（HPV）在高危人群中已知会抑制 I 型干扰素（IFN）信号通路，导致干扰素刺激基因（ISG）的转录，这些基因具有许多抗病毒功能。然而，HPV 对低危人群中各种 ISG 的作用的影响尚不完全清楚。我们旨在研究 2 型 HPV（HPV-2）转染的角质形成细胞中是否表达抗病毒 ISG。通过定量实时聚合酶链反应、western blot、免疫荧光和/或免疫组织化学评估 ISG 和 I 型 IFN 信号通路成分的 mRNA 和蛋白表达。与正常皮肤相比，HPV-2 阳性皮肤疣中的所有 ISG 的 mRNA 表达均显著降低（<0.05）。与空载体转染相比，转染显著下调了 ISG 和 I 型 IFN 信号通路成分的 mRNA 和蛋白表达，而 siRNA 转染显著上调了它们的表达（<0.05）。有趣的是，表没食子儿茶素没食子酸酯（EGCG）预处理上调了 ISG 和 I 型 IFN 信号通路成分的 mRNA 和蛋白表达，而转染则显著下调了它们的表达（<0.05）。我们的结果表明，EGCG 是预防皮肤疣的潜在候选药物。
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