Lee Hwa, Park Ho-Yong, Jeong Tae-Sook
Industrial Bio-materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
Life (Basel). 2021 Feb 15;11(2):147. doi: 10.3390/life11020147.
Pheophorbide a is a chlorophyll metabolic breakdown product. This study investigated the antiwrinkle effect of pheophorbide a (PA) and its derivatives, including pyropheophorbide a (PyroPA) and pyropheophorbide a methyl ester (PyroPA-ME), on ultraviolet (UV) B-stimulated CCD-986sk fibroblasts. PA, PyroPA, and PyroPA-ME effectively suppressed reactive oxygen species accumulation in UVB-exposed CCD-986sk fibroblasts. All three pheophorbides also reduced UVB-induced matrix metalloproteinase (MMP)-1 secretion and mRNA expression of , , and . Treatment with pheophorbides resulted in increased procollagen synthesis, and this required enhancement of procollagen type I C-peptide content and mRNA expression of collagen type I alpha 1 () and in CCD-986sk cells. These antiwrinkle effects were more potent with PA and PyroPA than with PyroPA-ME. Furthermore, PA and PyroPA suppressed UVB-induced phosphorylation of extracellular signal-regulated protein kinase and c-Jun N-terminal kinase but not p38. Moreover, all three pheophorbides inhibited NF-κB p65 phosphorylation. Therefore, these pheophorbides, especially PA and PyroPA, can be used as antiwrinkle agents, and PA- or PyroPA-rich natural resources can be used in functional cosmetics.
脱镁叶绿酸a是叶绿素的代谢分解产物。本研究调查了脱镁叶绿酸a（PA）及其衍生物，包括焦脱镁叶绿酸a（PyroPA）和焦脱镁叶绿酸a甲酯（PyroPA-ME），对紫外线（UV）B刺激的CCD-986sk成纤维细胞的抗皱作用。PA、PyroPA和PyroPA-ME有效抑制了UVB照射的CCD-986sk成纤维细胞中活性氧的积累。所有三种脱镁叶绿酸还降低了UVB诱导的基质金属蛋白酶（MMP）-1的分泌以及 、 和 的mRNA表达。用脱镁叶绿酸处理导致前胶原合成增加，这需要增强CCD-986sk细胞中I型前胶原C肽含量以及I型胶原α1（）和 的mRNA表达。PA和PyroPA的这些抗皱作用比PyroPA-ME更强。此外，PA和PyroPA抑制UVB诱导的细胞外信号调节蛋白激酶和c-Jun氨基末端激酶的磷酸化，但不抑制p38。而且，所有三种脱镁叶绿酸均抑制NF-κB p65的磷酸化。因此，这些脱镁叶绿酸，尤其是PA和PyroPA，可作为抗皱剂，富含PA或PyroPA的天然资源可用于功能性化妆品。
