Kim A-Ju, Park Jung Eun, Cho Yeong Hee, Lim Do Sung, Lee Jung Sup
Department of Biomedical Science, College of Natural Sciences, Chosun University, Gwangju 61452, Korea.
Department of Integrative Biological Sciences & BK21 FOUR Educational Research Group for Age-associated Disorder Control Technology, Chosun University, Gwangju 61452, Korea.
Life (Basel). 2021 Feb 20;11(2):162. doi: 10.3390/life11020162.
Skin aging, characterized by hyperpigmentation, inflammation, wrinkles, and skin cancer, is influenced by intrinsic and extrinsic factors with synergistic effects. Autophagy maintains the homeostatic balance between the degradation, synthesis, and recycling of cellular proteins and organelles, and plays important roles in several cellular and biological processes, including aging. The compound 7-methylsulfinylheptyl isothiocyanate (7-MSI) is a sulfur-containing phytochemical produced by various plants, particularly cruciferous vegetables, with reported anti-inflammatory properties and a role in pathogen defense; however, its effects on skin whitening have not been studied in detail. The purpose of this study was to observe the effects of 7-MSI on skin whitening and autophagy in cultured murine melanoma (B16-F1) cells. Western blotting was used to evaluate the impact of 7-MSI on melanogenesis-, tyrosinase-, and autophagy-associated proteins. The levels of the melanogenesis-associated protein's microphthalmia-associated transcription factor (MITF) and tyrosinase and tyrosinase-related protein-1 were decreased by treatment with 7-MSI under melanogenesis induction. Melanin synthesis also decreased by approximately 63% after treatment with 7-MSI for 73 h, compared with that non-treated controls. In addition, autophagosome formation and the expression levels of the autophagy-related proteins mTOR, p-mTOR, Beclin-1, Atg12, and LC3 were higher in 7-MSI-treated B16-F1 cells than in non-treated cells. These results indicate that 7-MSI can inhibit melanin synthesis in B16-F1 cells by suppressing melanogenesis and autophagy activation and thus can potentially be used as a novel multifunctional cosmetic agent.
皮肤老化的特征为色素沉着、炎症、皱纹和皮肤癌，受内在和外在因素影响且具有协同作用。自噬维持细胞蛋白质和细胞器降解、合成与循环之间的稳态平衡，并在包括衰老在内的多个细胞和生物学过程中发挥重要作用。化合物7-甲基亚磺酰基庚基异硫氰酸酯（7-MSI）是多种植物，特别是十字花科蔬菜产生的一种含硫植物化学物质，具有抗炎特性且在病原体防御中发挥作用；然而，其对皮肤美白的影响尚未得到详细研究。本研究的目的是观察7-MSI对培养的小鼠黑色素瘤（B16-F1）细胞皮肤美白和自噬的影响。采用蛋白质免疫印迹法评估7-MSI对黑素生成、酪氨酸酶和自噬相关蛋白的影响。在黑素生成诱导条件下，用7-MSI处理后，黑素生成相关蛋白小眼畸形相关转录因子（MITF）、酪氨酸酶和酪氨酸酶相关蛋白-1的水平降低。与未处理的对照组相比，用7-MSI处理73小时后，黑色素合成也减少了约63%。此外，7-MSI处理的B16-F1细胞中自噬体形成以及自噬相关蛋白mTOR、p-mTOR、Beclin-1、Atg12和LC3的表达水平高于未处理的细胞。这些结果表明，7-MSI可通过抑制黑素生成和自噬激活来抑制B16-F1细胞中的黑色素合成，因此有可能用作一种新型多功能化妆品原料。
