Persister Cells Form in the Plant Pathogen subsp. under Different Stress Conditions.

Citrus canker disease, caused by the bacterium subsp. is a constant threat to citrus-producing areas. Since it has no cure, agricultural practices to restrain its dissemination are essential to reduce the economic damage. Hence, increased knowledge of the basic aspects of biology could lead to more efficient management practices that can eliminate dormant bacteria in the field. The dormant cells, also referred to as persisters, are phenotypic variants with lowered metabolism, which in turn leads to tolerance to antimicrobials and undermines existing control approaches. We show here that forms persisters, identifying triggers for this phenotype, including antibiotics, high temperature, and metals (copper and zinc), which increase persistence rates by 10-100 times. The antioxidant N-acetylcysteine reduced copper and zinc-induced persisters, but not those induced by tetracycline, indicating that oxidative stress may be an important inducer of persistence. In addition, we found that metabolism-independent drugs like cisplatin and mitomycin C are able to eliminate persistent cells, as well as copper, at high concentrations. Specific amino acids like proline and isoleucine interfered with the physiological balance of the dormancy in , stimulating or preventing persister resuscitation. Taken together, we discover chemicals that can induce, wake, and kill persister cells; these results provide insights that should be considered for more efficient integrated control management in the field.