Campos-García Tonatiu, Molina-Torres Jorge
Department of Biotecnología y Bioquímica, CINVESTAV Unidad Irapuato, Irapuato, Guanajuato 36924, Mexico.
Metabolites. 2021 Feb 27;11(3):143. doi: 10.3390/metabo11030143.
Alkamides have been observed to interact in different ways in several superior organisms and have been used in traditional medicine in many countries e.g., to relieve pain. Previous studies showed that affinin when applied to other plant species induces prominent changes in the root architecture and induces transcriptional adjustments; however, little is known about the metabolic pathways recruited by plants in response to alkamides. Previous published work with Arabidopsis seedlings treated in vitro with affinin at 50 µM significantly reduced primary root length. In tomato seedlings, that concentration did not reduce root growth but increase the number and length of lateral roots. Non-targeted metabolomic analysis by Gas Chromatography couplet to Mass Spectrometry (GC/EIMS) showed that, in tomato seedlings, affinin increased the accumulation of several metabolites leading to an enrichment of several metabolic pathways. Affinin at 100 µM alters the accumulation of metabolites such as organic acids, amino acids, sugars, and fatty acids. Finally, our results showed a response possibly associated with nitrogen, GABA shunt and serine pathways, in addition to a possible alteration in the mitochondrial electron transport chain (ETC), interesting topics to understand the molecular and metabolic mechanisms in response to alkamide in plants.
已观察到链状酰胺在几种高等生物中以不同方式相互作用，并在许多国家的传统医学中得到应用，例如用于缓解疼痛。先前的研究表明，将阿菲宁应用于其他植物物种时，会引起根系结构的显著变化并诱导转录调整；然而，关于植物响应链状酰胺所招募的代谢途径知之甚少。先前发表的关于用50 μM阿菲宁体外处理拟南芥幼苗的研究表明，主根长度显著缩短。在番茄幼苗中，该浓度并未降低根系生长，反而增加了侧根的数量和长度。通过气相色谱联用质谱法（GC/EIMS）进行的非靶向代谢组学分析表明，在番茄幼苗中，阿菲宁增加了几种代谢物的积累，导致几种代谢途径富集。100 μM的阿菲宁会改变有机酸、氨基酸、糖类和脂肪酸等代谢物的积累。最后，我们的结果表明，除了线粒体电子传递链（ETC）可能发生改变外，还可能存在与氮、GABA分流和丝氨酸途径相关的反应，这些都是理解植物对链状酰胺响应的分子和代谢机制的有趣课题。
