Laboratorio de Investigación en Microbiología, Dirección de Investigación y Enseñanza, Hospital Juárez de México, Av. Instituto Politécnico Nacional, 5160 México D.F., México.
Pharm Biol. 2013 Aug;51(8):1035-9. doi: 10.3109/13880209.2013.775161. Epub 2013 Jun 5.
Heliopsis longipes (A. Gray) Blake (Asteraceae), commonly known in Mexico as "chilcuage" or "chilcuan", is widely used as an analgesic and anesthetic agent. Affinin, the major metabolite of this plant, and the ethanol extract of the plant have shown antinociceptive properties in mice. H. longipes plant produces a complex mixture of antioxidant chlorophylls and polyamines as well as a number of possible antimutagens.
The current study evaluated the potential utilization of the natural product affinin isolated from H. longipes ethanol extract as an antimutagenic and possibly anticarcinogenic agent.
The Ames assay was used to assess the mutagenic properties of affinin (12.5, 25 and 50 µg/plate) that was added to several mutagens with or without S9 metabolic activation in Salmonella typhimurium (TA98, TA100 and TA102 strains).
Heliopsis longipes extract and affinin were not toxic as a reduction in the number of His⁺ revertant bacteria colonies. Affinin (25 and 50 µg/plate) significantly reduced the frameshift mutations that were generated by 2-aminoanthracene (2AA) (40%) and reduced the oxidative DNA damage generated by norfloxacin (NOR) (37-50%). Affinin possessed antioxidant properties that were able to reduce 2AA- and NOR-induced mutations in S. typhimurium TA98 and TA102, respectively.
Affinin, the principal metabolite of H. longipes, is not mutagenic and possesses antimutagenic activity. These plants are currently used to treat some pain symptoms in Mexico; and antimutagen activity determined could be important to treat some pain symptoms related to antiradical activity.
墨西哥俗称“chilcuage”或“chilcuan”的黄鹌菜（ Asteraceae ）是一种广泛应用于止痛和麻醉的草药。这种植物的主要代谢产物 affinin 以及植物乙醇提取物已被证明在小鼠体内具有镇痛作用。黄鹌菜植物产生复杂的抗氧化叶绿素和多胺混合物，以及许多可能的抗诱变剂。
本研究评估了从黄鹌菜乙醇提取物中分离得到的天然产物 affinin 作为一种抗诱变剂和潜在的抗癌剂的潜在用途。
使用 Ames 试验评估 affinin（12.5、25 和 50μg/平板）的诱变特性，该物质在加入几种诱变剂时（有或没有 S9 代谢激活），在鼠伤寒沙门氏菌（TA98、TA100 和 TA102 菌株）中进行评估。
黄鹌菜提取物和 affinin 没有毒性，因为不会减少 His+回复突变菌的菌落数。affinin（25 和 50μg/平板）显著减少了 2-氨基蒽（2AA）产生的移码突变（40%），并减少了诺氟沙星（NOR）产生的氧化 DNA 损伤（37-50%）。affinin 具有抗氧化特性，能够分别减少 2AA 和 NOR 诱导的 S. typhimurium TA98 和 TA102 突变。
黄鹌菜的主要代谢产物 affinin 没有致突变性，并且具有抗诱变活性。这些植物目前被用于治疗墨西哥的一些疼痛症状；而所确定的抗突变活性可能对治疗与抗自由基活性相关的一些疼痛症状很重要。
