Abdel-Farid Ibrahim Bayoumi, Marghany Marwa Radawy, Rowezek Mohamed Mahmoud, Sheded Mohamed Gabr
Biology Department, College of Science, Jouf University, Sakaka P.O. Box 2014, Saudi Arabia.
Botany Department, Faculty of Science, Aswan University, Aswan 81528, Egypt.
Plants (Basel). 2020 Nov 23;9(11):1626. doi: 10.3390/plants9111626.
Seeds germination and seedlings growth of and were monitored in in vitro and in vivo experiments after application of different concentrations of NaCl (25, 50, 100 and 200 mM). Photosynthetic pigments content and the biochemical responses of and were assessed. Salinity stress slightly delayed the seeds germination rate and significantly reduced the percentage of germination as well as shoot length under the highest salt concentration (200 mM) in cucumber. Furthermore, root length was decreased significantly in all treatments. Whereas, in tomato, a prominent delay in seeds germination rate, the germination percentage and seedlings growth (shoot and root lengths) were significantly influenced under all concentrations of NaCl. Fresh and dry weights were reduced prominently in tomato compared to cucumber. Photosynthetic pigments content was reduced but with pronounced decreasing in tomato compared to cucumber. Secondary metabolites profiling in both plants under stress was varied from tomato to cucumber. The content of saponins, proline and total antioxidant capacity was reduced more prominently in tomato as compared to cucumber. On the other hand, the content of phenolics and flavonoids was increased in both plants with pronounced increase in tomato particularly under the highest level of salinity stress. The metabolomic profiling in stressful plants was significantly influenced by salinity stress and some bioactive secondary metabolites was enhanced in both cucumber and tomato plants. The enhancement of secondary metabolites under salinity stress may explain the tolerance and sensitivity of cucumber and tomato under salinity stress. The metabolomic evaluation combined with multivariate data analysis revealed a similar mechanism of action of plants to mediate stress, with variant level of this response in both plant species. Based on these results, the effect of salinity stress on seeds germination, seedlings growth and metabolomic content of plants was discussed in terms of tolerance and sensitivity of plants to salinity stress.
在施加不同浓度的NaCl（25、50、100和200 mM）后，在体外和体内实验中监测了黄瓜和番茄的种子萌发及幼苗生长情况。评估了黄瓜和番茄的光合色素含量及生化反应。盐胁迫略微延迟了黄瓜种子的萌发速率，并在最高盐浓度（200 mM）下显著降低了发芽率和茎长。此外，在所有处理中黄瓜根长均显著降低。而在番茄中，在所有NaCl浓度下种子萌发速率、发芽率和幼苗生长（茎长和根长）均受到显著影响。与黄瓜相比，番茄的鲜重和干重显著降低。光合色素含量降低，但与黄瓜相比，番茄的降低更为明显。胁迫下两种植物的次生代谢产物谱在番茄和黄瓜之间存在差异。与黄瓜相比，番茄中皂苷、脯氨酸和总抗氧化能力的含量降低更为显著。另一方面，两种植物中酚类和黄酮类化合物的含量均增加，尤其是在最高盐胁迫水平下，番茄中的增加更为明显。盐胁迫显著影响了胁迫植物的代谢组学谱，黄瓜和番茄植株中一些生物活性次生代谢产物均有所增加。盐胁迫下次生代谢产物的增加可能解释了黄瓜和番茄在盐胁迫下的耐受性和敏感性。代谢组学评估结合多变量数据分析揭示了植物介导胁迫的类似作用机制，两种植物物种的这种反应水平有所不同。基于这些结果，从植物对盐胁迫的耐受性和敏感性方面讨论了盐胁迫对植物种子萌发、幼苗生长和代谢组学含量的影响。
