某些药用植物物种（×、、、和）的体外培养作为抗氧化剂的丰富潜在来源——通过CUPRAC和QUENCHER-CUPRAC测定法进行评估
In Vitro Cultures of Some Medicinal Plant Species ( × , , , and ) as a Rich Potential Source of Antioxidants-Evaluation by CUPRAC and QUENCHER-CUPRAC Assays.
作者信息
Dziurka Michał, Kubica Paweł, Kwiecień Inga, Biesaga-Kościelniak Jolanta, Ekiert Halina, Abdelmohsen Shaimaa A M, Al-Harbi Fatemah F, Elansary Diaa O, Elansary Hosam O, Szopa Agnieszka
机构信息
Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, ul. Niezapominajek 21, 30-239 Kraków, Poland.
Chair and Department of Pharmaceutical Botany, Jagiellonian University, Medical College, Medyczna 9, 30-688 Kraków, Poland.
出版信息
Plants (Basel). 2021 Feb 27;10(3):454. doi: 10.3390/plants10030454.
Comparative estimations of the antioxidant activity of methanolic extracts from biomasses of different types of in vitro cultures of , and and also from plant raw materials were performed. The antioxidant measurements were based on the modern assays-cupric ion reducing antioxidant capacity (CUPRAC) and quick, easy, new, cheap, and reproducible CUPRAC (QUENCHER-CUPRAC). The total extractable antioxidants (CUPRAC assay) ranged from 10.4 to 49.7 mmol (100 g) of dry weight (DW) expressed as Trolox equivalent antioxidant capacity (TEAC), and the global antioxidant response (QUENCHER-CUPRAC assay) ranged from 16.0 to 79.1 mmol (100 g) DW for in vitro cultures, whereas for plant raw materials the total extractable antioxidants ranged from 20.9 to 69.5 mmol (100 g) DW, and the global antioxidant response ranged from 67.2 to 97.8 mmol (100 g) DW. Finally, the in vitro cultures could be regarded as an antioxidant-rich alternative resource for the pharmaceutical, health food and cosmetics industries.
对来自不同类型的体外培养物、以及植物原料的甲醇提取物的抗氧化活性进行了比较评估。抗氧化剂的测定基于现代分析方法——铜离子还原抗氧化能力（CUPRAC）和快速、简便、新颖、廉价且可重复的CUPRAC（QUENCHER-CUPRAC）。以Trolox等效抗氧化能力（TEAC）表示，总可提取抗氧化剂（CUPRAC分析）范围为10.4至49.7 mmol（100 g）干重（DW），对于体外培养物，整体抗氧化反应（QUENCHER-CUPRAC分析）范围为16.0至79.1 mmol（100 g）DW，而对于植物原料，总可提取抗氧化剂范围为20.9至69.5 mmol（100 g）DW，整体抗氧化反应范围为67.2至97.8 mmol（100 g）DW。最后，体外培养物可被视为制药、保健食品和化妆品行业富含抗氧化剂的替代资源。
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