Everson R B, Wehr C M, Erexson G L, MacGregor J T
Epidemiology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
J Natl Cancer Inst. 1988 Jun 1;80(7):525-9. doi: 10.1093/jnci/80.7.525.
Recent studies have demonstrated that in the absence of spleen function, frequencies of micronuclei (Howell-Jolly bodies) in peripheral blood rbcs can be used to measure in vivo cytogenetic damage. Among 20 subjects studied greater than or equal to 6 months after splenectomy, 1 had a frequency of micronucleated rbcs more than an order of magnitude higher than rates for the others. Initial data suggested that this subject was mildly folate-depleted, and a therapeutic trial with folate rapidly reduced the frequency of micronucleated rbcs to normal values. These observations suggest a need to evaluate further the contribution of mild levels of folate depletion to spontaneous chromosomal damage. The approach used here provides a sensitive index of clastogenic damage and offers unique opportunities for investigating the determinants of cytogenetic damage in humans.
最近的研究表明，在脾脏功能缺失的情况下，外周血红细胞中的微核（豪-乔小体）频率可用于测量体内细胞遗传损伤。在20名脾切除术后6个月及以上的研究对象中，有1名的微核红细胞频率比其他对象高出一个数量级以上。初步数据表明该对象存在轻度叶酸缺乏，叶酸治疗试验迅速将微核红细胞频率降至正常值。这些观察结果表明需要进一步评估轻度叶酸缺乏对自发染色体损伤的影响。这里使用的方法提供了一种灵敏的致断裂损伤指标，并为研究人类细胞遗传损伤的决定因素提供了独特的机会。
