Oviedo Maria, Montoya Yuliet, Agudelo Wilson, García-García Alejandra, Bustamante John
Grupo de Dinámica Cardiovascular, Centro de Bioingeniería, Universidad Pontificia Bolivariana, Medellín 050031, Colombia.
Laboratorio de Síntesis and Modificación de Nanoestructuras and Materiales Bidimensionales, Centro de Investigación en Materiales Avanzados, Chihuahua 31136, Mexico.
Polymers (Basel). 2021 Dec 10;13(24):4320. doi: 10.3390/polym13244320.
Tissue engineering has focused on the development of biomaterials that emulate the native extracellular matrix. Therefore, the purpose of this research was oriented to the development of nanofibrillar bilayer membranes composed of polycaprolactone with low and medium molecular weight chitosan, evaluating their physicochemical and biological properties. Two-bilayer membranes were developed by an electrospinning technique considering the effect of chitosan molecular weight and parameter changes in the technique. Subsequently, the membranes were evaluated by scanning electron microscopy, Fourier transform spectroscopy, stress tests, permeability, contact angle, hemolysis evaluation, and an MTT test. From the results, it was found that changes in the electrospinning parameters and the molecular weight of chitosan influence the formation, fiber orientation, and nanoarchitecture of the membranes. Likewise, it was evidenced that a higher molecular weight of chitosan in the bilayer membranes increases the stiffness and favors polar anchor points. This increased Young's modulus, wettability, and permeability, which, in turn, influenced the reduction in the percentage of cell viability and hemolysis. It is concluded that the development of biomimetic bilayer nanofibrillar membranes modulate the physicochemical properties and improve the hemolytic behavior so they can be used as a hemocompatible biomaterial.
组织工程学一直专注于开发能够模拟天然细胞外基质的生物材料。因此，本研究的目的是开发由低分子量和中分子量壳聚糖与聚己内酯组成的纳米纤维双层膜，并评估其物理化学和生物学特性。考虑到壳聚糖分子量的影响以及该技术中参数的变化，通过静电纺丝技术制备了两种双层膜。随后，通过扫描电子显微镜、傅里叶变换光谱、应力测试、渗透性、接触角、溶血评估和MTT试验对膜进行了评估。结果发现，静电纺丝参数和壳聚糖分子量的变化会影响膜的形成、纤维取向和纳米结构。同样，有证据表明双层膜中较高分子量的壳聚糖会增加膜的硬度并有利于极性锚点。这提高了杨氏模量、润湿性和渗透性，进而影响了细胞活力百分比和溶血率的降低。结论是仿生双层纳米纤维膜的开发调节了物理化学性质并改善了溶血行为，因此它们可以用作血液相容性生物材料。
