Department of Pharmacology and Physiology, University of Calgary, Calgary, Alberta, Canada.
Snyder Institute for Chronic Diseases, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
Science. 2021 Mar 5;371(6533). doi: 10.1126/science.abe0595.
Most multicellular organisms have a major body cavity that harbors immune cells. In primordial species such as purple sea urchins, these cells perform phagocytic functions but are also crucial in repairing injuries. In mammals, the peritoneal cavity contains large numbers of resident GATA6 macrophages, which may function similarly. However, it is unclear how cavity macrophages suspended in the fluid phase (peritoneal fluid) identify and migrate toward injuries. In this study, we used intravital microscopy to show that cavity macrophages in fluid rapidly form thrombus-like structures in response to injury by means of primordial scavenger receptor cysteine-rich domains. Aggregates of cavity macrophages physically sealed injuries and promoted rapid repair of focal lesions. In iatrogenic surgical situations, these cavity macrophages formed extensive aggregates that promoted the growth of intra-abdominal scar tissue known as peritoneal adhesions.
大多数多细胞生物都有一个容纳免疫细胞的主要体腔。在原始物种如紫色海胆中，这些细胞具有吞噬功能，但在修复损伤方面也至关重要。在哺乳动物中，腹腔内含有大量的常驻 GATA6 巨噬细胞，它们可能具有类似的功能。然而，目前尚不清楚悬浮在液体相（腹腔液）中的腔巨噬细胞如何识别并迁移到损伤部位。在这项研究中，我们使用活体显微镜观察到，腔巨噬细胞在流体中迅速形成血栓样结构，这是通过原始清道夫受体富含半胱氨酸结构域实现的。巨噬细胞聚集物物理性地封闭了损伤部位，并促进了局灶性病变的快速修复。在医源性手术情况下，这些腔巨噬细胞形成了广泛的聚集物，促进了称为腹腔粘连的腹腔内疤痕组织的生长。
