Laboratory of Animal Science, Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.
Laboratory of Animal Science, Department of Applied Biological Sciences, Faculty of Agriculture, Setsunan University, Hirakata, Osaka, Japan.
Biosci Biotechnol Biochem. 2021 Apr 24;85(5):1235-1242. doi: 10.1093/bbb/zbab029.
The role of Fusobacterium nucleatum, often associated with intestinal diseases, in the remission of dextran sulfate sodium (DSS)-induced colitis was investigated. Female mice were divided into groups DC (DSS control) and DF (DSS + F. nucleatum). F. nucleatum (1.0 × 1010 cfu/mouse/day) in phosphate-buffered saline (PBS) was orally given to DF, while DC had PBS only. All mice had DSS in drinking water. In Experiment 1, mice underwent 2 inflammation phases, an in-between recovery phase and had their disease activity indices (DAI) calculated. Experiment 2 was similarly conducted, except that mice were dissected 3 days postrecovery, and had blood and colonic mucosal samples collected. In Experiment 1, DF had significantly (P < .05) higher DAI than DC, during the recovery and 2nd inflammation phases. In Experiment 2, genus Bacteroides was significantly (P < .05) higher and family Lachnospiraceae significantly lower in cecal mucosa-associated microbiota of DF than in that of DC. We concluded that F. nucleatum can impede colitis remission.
研究了常与肠道疾病相关的具核梭杆菌（Fusobacterium nucleatum）在葡聚糖硫酸钠（Dextran sulfate sodium，DSS）诱导的结肠炎缓解中的作用。将雌性小鼠分为 DC（DSS 对照组）和 DF（DSS+F. nucleatum 组）。DF 组用磷酸盐缓冲盐水（Phosphate-buffered saline，PBS）给予 F. nucleatum（1.0×1010 cfu/只/天），而 DC 组仅给予 PBS。所有小鼠的饮用水中都含有 DSS。在实验 1 中，小鼠经历了 2 个炎症阶段、一个中间恢复期，并计算了疾病活动指数（Disease activity index，DAI）。实验 2 以类似的方式进行，只是在恢复期后 3 天对小鼠进行解剖，并采集血液和结肠黏膜样本。在实验 1 中，DF 组在恢复期和第 2 次炎症期的 DAI 明显（P<0.05）高于 DC 组。在实验 2 中，DF 组的拟杆菌属（Bacteroides）明显（P<0.05）高于 DC 组，而lachnospiraceae 家族明显（P<0.05）低于 DC 组。我们得出结论，具核梭杆菌可以阻碍结肠炎的缓解。
