Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt.
J Appl Microbiol. 2022 Mar;132(3):2020-2033. doi: 10.1111/jam.15221. Epub 2021 Nov 3.
This study aims to use fermentation waste of ethanol production (solid and liquid) for riboflavin and recycling of bacterial biomass as biofertilizers to enhance the growth of some oily crop plants.
Out of 10 yeast isolates from fresh milk, Clavispora lusitaniae ASU 33 (MN583181) was able to ferment different concentrations of glucose (2.5%, 5%, 7.5%, 10%, 15% and 20%) into ethanol with high efficiency at 10%. Among seven non-Lactobacillus bacterial isolates recovered from cheese samples, two bacterial isolates Bacillus subtlis-SR2 (MT002768) and Novosphingobium panipatense-SR3 (MT002778) were selected for their high riboflavin production. Different media (control medium, fermentation waste medium and a mixture of the fermentation waste medium and control medium [1:1]) were used for riboflavin production. These media were inoculated by a single or mixture of B. subtlis-SR2, N. panipatense-SR3. The addition of the waste medium of ethanol production to the control medium (1:1) had a stimulatory effect on riboflavin production whether inoculated with either a single strain or a mixture of B. subtlis-SR2 and N. panipatense-SR3. A mixture of fermentation waste and control media inoculated with N. panipatense produced a high riboflavin yield in comparison with other media. Inoculation of Zea mays and Ocimum basilicum plants with either the bacterial biomass waste of riboflavin production (B. subtlis or N. panipatense) or a mixture of B. subtlis and N. panipatense) shows a stimulatory effect on the plant growth in comparison with control (uninoculated plants).
These results demonstrate the possibility of minimizing the cost of riboflavin and biofertilizer manufacturing via interlinking ethanol and riboflavin with the biofertilizer production technology.
This study outlines the methods of evaluating the strength of spent media by applying procedures developed in the vitamin production industries. Furthermore, bacterial biomass waste can act as an environmentally friendly alternative for agrochemicals.
本研究旨在利用乙醇生产的发酵废物（固体和液体）生产核黄素，并回收细菌生物质作为生物肥料，以促进一些含油作物的生长。
从新鲜牛奶中分离出的 10 株酵母中，Clavispora lusitaniae ASU 33（MN583181）能够在 10%的浓度下高效发酵不同浓度的葡萄糖（2.5%、5%、7.5%、10%、15%和 20%）生成乙醇。从奶酪样品中分离出的 7 株非乳酸菌中，选择了 2 株细菌 Bacillus subtlis-SR2（MT002768）和 Novosphingobium panipatense-SR3（MT002778），因为它们具有较高的核黄素产量。不同的培养基（对照培养基、发酵废物培养基和发酵废物培养基与对照培养基的混合物[1:1]）用于核黄素生产。这些培养基分别由单株或 Bacillus subtlis-SR2 和 Novosphingobium panipatense-SR3 的混合物接种。将乙醇生产的废培养基添加到对照培养基（1:1）中，无论是单独接种一株菌还是混合接种 Bacillus subtlis-SR2 和 Novosphingobium panipatense-SR3，都对核黄素的生产有刺激作用。与其他培养基相比，用发酵废物和对照培养基混合物接种 Novosphingobium panipatense 产生了较高的核黄素产量。用核黄素生产的细菌生物量废物（Bacillus subtlis 或 Novosphingobium panipatense）或 Bacillus subtlis 和 Novosphingobium panipatense 的混合物接种玉米和罗勒植物，与对照（未接种植物）相比，对植物生长有刺激作用。
这些结果表明，通过将乙醇和核黄素与生物肥料生产技术相联系，可以降低核黄素和生物肥料生产成本。
本研究概述了通过应用维生素生产行业开发的程序来评估废培养基强度的方法。此外，细菌生物量废物可以作为一种环保的农用化学品替代品。
